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Literature summary for 3.2.2.15 extracted from
- Hypoxanthine in deoxyribonucleic acid: generation by heat-induced hydrolysis of adenine residues and release in free form by a deoxyribonucleic acid glycosylase from calf thymus (1980), Biochemistry, 19, 6005-6011.
General Stability
| General Stability | Organism |
|---|---|
| fully active in presence of 1-5 mM EDTA | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | higher concentrations, above 10 mM CaCl2 | Bos taurus |  |
| DNA | markedly inhibited by low concentrations of native DNA | Bos taurus | |
| KCl | 2fold inhibited | Escherichia coli | |
| Mg2+ | higher concentrations, above 20 mM MgCl2 | Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0005 | - |
poly(dAdT) | - |
Bos taurus | |
| 0.0009 | - |
poly-dC | - |
Bos taurus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | activity 4fold stimulated by 0.1 M KCl | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
- |
Escherichia coli |
| 31000 | - |
SDS-PAGE | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | Escherichia coli | - |
? | - |
? | |
| DNA + H2O | Homo sapiens | - |
? | - |
? | |
| DNA + H2O | Bos taurus | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
calf | - |
| Escherichia coli | - |
- |
- |
| Homo sapiens | - |
human, normal and progeroid cells | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| skin fibroblast | - |
Homo sapiens | - |
| skin fibroblast | - |
Bos taurus | - |
| thymus | - |
Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | - |
Escherichia coli | hypoxanthine + ? | - |
? | |
| DNA + H2O | preference for double-stranded substrates | Bos taurus | hypoxanthine + ? | liberated from DNA directly as free base, direct cleavage of the base-sugar bonds of dIMP residues in the polymer | ? | |
| DNA + H2O | - |
Escherichia coli | ? | - |
? | |
| DNA + H2O | - |
Homo sapiens | ? | - |
? | |
| DNA + H2O | - |
Bos taurus | ? | - |
? | |
| additional information | DNA repair by initiating a base excision-repair at a damaged or misincorporated residue | Escherichia coli | ? | - |
? | |
| additional information | DNA repair by initiating a base excision-repair at a damaged or misincorporated residue | Homo sapiens | ? | - |
? | |
| additional information | DNA repair by initiating a base excision-repair at a damaged or misincorporated residue | Bos taurus | ? | - |
? | |
| poly(dAdT) + H2O | - |
Bos taurus | ? | - |
? | |
| poly-dC + H2O | - |
Bos taurus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| bovine hypoxanthine-DNA glycosylase | - |
Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | 7.8 | - |
Bos taurus |
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Release 2023.1 (January 2023)
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