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Literature summary for 3.2.1.B34 extracted from

  • Anbarasan, S.; Timoharju, T.; Barthomeuf, J.; Pastinen, O.; Rouvinen, J.; Leisola, M.; Turunen, O.
    Effect of active site mutation on pH activity and transglycosylation ofSulfolobus acidocaldarius beta-glycosidase (2015), J. Mol. Catal. B, 118, 62-69.
No PubMed abstract available
Search for more literature for 3.2.1.B34

Protein Variants

Protein Variants Comment Organism
N211D mutation changes the polarity close to the putative acid/base catalyst E209, leads to decrease in activity Sulfolobus acidocaldarius
V212D mutation changes the polarity close to the putative acid/base catalyst E209, shifts the pH-activity profile towards acidic pH with both lactose and cellobiose as substrates and leads to decrease in activity Sulfolobus acidocaldarius
V212T mutation changes the polarity close to the putative acid/base catalyst E209, shifts the pH-activity profile towards acidic pH with both lactose and cellobiose as substrates. Although V212T increases 6fold the Kmvalue with cellobiose, the mutant shows higher specific activity in high substrate concentrations due to greatly reduced produc-tion of trisaccharide by V212T from cellobiose by transglycosylation Sulfolobus acidocaldarius

Inhibitors

Inhibitors Comment Organism Structure
D-galactose endproduct inhibition, inhibition of enzymeactivity was stronger with glucose than galactose Sulfolobus acidocaldarius
D-glucose endproduct inhibition, inhibition of enzyme activity is stronger with glucose than galactose Sulfolobus acidocaldarius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 lactose wild-type, Km value 101 mg/ml, mutant V212T, 75 mg/ml, respectively, pH 5.0, 50°C Sulfolobus acidocaldarius
additional information
-
 cellobiose wild-type, Km value 17 mg/ml, mutant V212T, 102 mg/ml, respectively, pH 5.0, 50°C Sulfolobus acidocaldarius

Organism

Organism UniProt Comment Textmining
Sulfolobus acidocaldarius P14288
-
-
Sulfolobus acidocaldarius DSM 639 P14288
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
40
-
 mutant V212D, at 100 g/l cellobiose, pH 5.0, 50°C Sulfolobus acidocaldarius
62
-
 mutant N211D, at 100 g/l cellobiose, pH 5.0, 50°C Sulfolobus acidocaldarius
173
-
 mutant V212D, at 100 g/l lactose, pH 5.0, 50°C Sulfolobus acidocaldarius
189
-
 wild-type, at 100 g/l cellobiose, pH 5.0, 50°C Sulfolobus acidocaldarius
262
-
 mutant N211D, at 100 g/l lactose, pH 5.0, 50°C Sulfolobus acidocaldarius
278
-
 wild-type, at 100 g/l lactose, pH 5.0, 50°C Sulfolobus acidocaldarius
321
-
 mutant V212T, at 100 g/l cellobiose, pH 5.0, 50°C Sulfolobus acidocaldarius
383
-
 mutant V212T, at 100 g/l lactose, pH 5.0, 50°C Sulfolobus acidocaldarius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + H2O
-
 Sulfolobus acidocaldarius 2 D-glucose
-
 ?
cellobiose + H2O
-
 Sulfolobus acidocaldarius DSM 639 2 D-glucose
-
 ?
lactose + H2O
-
 Sulfolobus acidocaldarius D-galactose + D-glucose
-
 ?
lactose + H2O
-
 Sulfolobus acidocaldarius DSM 639 D-galactose + D-glucose
-
 ?
additional information enzyme shows transglycosylation activity, producing close to 40% and 60% amounts of trisaccharide compared to the glucose in one h reaction with 100 and 200 g/l cellobiose, respectively Sulfolobus acidocaldarius ?
-
 ?
additional information enzyme shows transglycosylation activity, producing close to 40% and 60% amounts of trisaccharide compared to the glucose in one h reaction with 100 and 200 g/l cellobiose, respectively Sulfolobus acidocaldarius DSM 639 ?
-
 ?

Synonyms

Synonyms Comment Organism
BgaS
-
 Sulfolobus acidocaldarius

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
 mutant V212D, substrate cellobiose Sulfolobus acidocaldarius
80
-
 mutant V212D, substrate lactose Sulfolobus acidocaldarius
84
-
 mutant N211D, substrate cellobiose Sulfolobus acidocaldarius
90
-
 mutant V212D, substrate cellobiose Sulfolobus acidocaldarius
92
-
 wild-type, mutant N211D and V212T, substrate lactose Sulfolobus acidocaldarius
92
-
 wild-type, substrate cellobiose Sulfolobus acidocaldarius

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
92
-
 mutant V212T, 30 min, 50% residual activity Sulfolobus acidocaldarius