Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.B34 extracted from

  • Shin, K.C.; Choi, H.Y.; Seo, M.J.; Oh, D.K.
    Compound K production from red ginseng extract by beta-glycosidase from Sulfolobus solfataricus supplemented with alpha-L-arabinofuranosidase from Caldicellulosiruptor saccharolyticus (2015), PLoS One, 10, e0145876.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.B34

Application

Application Comment Organism
synthesis use of alpha-L-arabinofuranosidase from Caldicellulosiruptor saccharolyticus, EC 3.2.1.55, along with beta-glycosidase from Sulfolobus solfataricus to produce ginsenoside compound K from the protopanaxadiol-type ginsenosides in red-ginseng extract. The optimal reaction conditions are as follows: pH 6.0, 80°C, 2 U/ml Sulfolobus solfataricus enzyme, 3 U/ml Caldicellulosiruptor saccharolyticus enzyme, and 7.5 g/l protopanaxadiol-type ginsenosides. The enzymes produce 4.2 g/l ginsenoside compound K from 7.5 g/l ginsenosides in 12 h without other ginsenosides, with a molar yield of 100% and a productivity of 348 mg/l/h Saccharolobus solfataricus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus P22498
-
-
Saccharolobus solfataricus DSM 1617 P22498
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ginsenoside Rd + H2O
-
 Saccharolobus solfataricus ginsenoside K + D-glucopyranose
-
 ?
ginsenoside Rd + H2O
-
 Saccharolobus solfataricus DSM 1617 ginsenoside K + D-glucopyranose
-
 ?
additional information enzyme shows low activity on the alpha-L-arabinofuranoside linkages in ginsenosides Saccharolobus solfataricus ?
-
 ?
additional information enzyme shows low activity on the alpha-L-arabinofuranoside linkages in ginsenosides Saccharolobus solfataricus DSM 1617 ?
-
 ?

Synonyms

Synonyms Comment Organism
LACS
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
 assay at Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at Saccharolobus solfataricus