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Literature summary for 3.2.1.B34 extracted from

  • Noh, K.H.; Oh, D.K.
    Production of the rare ginsenosides compound K, compound Y, and compound Mc by a thermostable beta-glycosidase from Sulfolobus acidocaldarius (2009), Biol. Pharm. Bull., 32, 1830-1835.
    View publication on PubMed
Search for more literature for 3.2.1.B34

Application

Application Comment Organism
synthesis enzyme is a potential producer of the rare ginsenosides compound K, compound Y, and compound Mc from the major ginsenosides Rb1, Rb2, Rc, and Rd Sulfolobus acidocaldarius

Organism

Organism UniProt Comment Textmining
Sulfolobus acidocaldarius P14288
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Sulfolobus acidocaldarius DSM 639 P14288
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-L-arabinoside + H2O 17% of the activity compared to 4-nitrophenyl beta-D-glucopyranoside Sulfolobus acidocaldarius 4-nitrophenol + L-arabinopyranose
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 ?
4-nitrophenyl alpha-L-arabinoside + H2O 17% of the activity compared to 4-nitrophenyl beta-D-glucopyranoside Sulfolobus acidocaldarius DSM 639 4-nitrophenol + L-arabinopyranose
-
 ?
4-nitrophenyl beta-D-galactopyranoside + H2O 28% of the activity compared to 4-nitrophenyl beta-D-glucopyranoside Sulfolobus acidocaldarius 4-nitrophenol + D-galactopyranose
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 ?
4-nitrophenyl beta-D-galactopyranoside + H2O 28% of the activity compared to 4-nitrophenyl beta-D-glucopyranoside Sulfolobus acidocaldarius DSM 639 4-nitrophenol + D-galactopyranose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Sulfolobus acidocaldarius 4-nitrophenol + D-glucopyranose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Sulfolobus acidocaldarius DSM 639 4-nitrophenol + D-glucopyranose
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 ?
4-nitrophenyl beta-L-arabinopyranoside + H2O 8% of the activity compared to 4-nitrophenyl beta-D-glucopyranoside Sulfolobus acidocaldarius 4-nitrophenol + L-arabinopyranose
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 ?
4-nitrophenyl beta-L-arabinopyranoside + H2O 8% of the activity compared to 4-nitrophenyl beta-D-glucopyranoside Sulfolobus acidocaldarius DSM 639 4-nitrophenol + L-arabinopyranose
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 ?
ginsenoside Rb1 + H2O
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 Sulfolobus acidocaldarius ginsenoside Rd + ?
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 ?
ginsenoside Rb1 + H2O
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 Sulfolobus acidocaldarius DSM 639 ginsenoside Rd + ?
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 ?
ginsenoside Rb2 + H2O hydrolysis of the 3-O-beta-D-glucopyranosyl-(1->2)-beta-D-glucopyranosyl linkage in panaxadiol, activity is higher than hydrolysis of the 20-O-alpha-L-arabinopyranosyl-(1->6)-beta-D-glucopyranose linkage in ginsenoside Y Sulfolobus acidocaldarius ginsenoside Y + D-glucopyranose
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 ?
ginsenoside Rc + H2O hydrolysis of the 3-O-beta-D-glucopyranosyl-(1->2)-beta-D-glucopyranosyl linkage in panaxadiol, activity is higher than hydrolysis of the 20-O-alpha-L-arabinopyranosyl-(1->6)-beta-D-glucopyranose linkage in ginsenoside Y Sulfolobus acidocaldarius ginsenoside Mc + D-glucopyranose
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 ?
ginsenoside Rd + H2O hydrolysis of the 3-O-beta-D-glucopyranosyl-(1->2)-beta-D-glucopyranosyl linkage in panaxadiol, activity is higher than hydrolysis of the 20-O-alpha-L-arabinopyranosyl-(1->6)-beta-D-glucopyranose linkage in ginsenoside Y Sulfolobus acidocaldarius ginsenoside K + D-glucopyranose the enzyme does not convert compound Mc to compound K due to no activity for the alpha-L-arabinofuranose linkage in 20-O-alpha-L-arabinofuranosyl-(1->6)-beta-D-glucopyranose ?
ginsenoside Y + H2O hydrolysis of alpha-L-arabinopyranose linkage in 20-O-alpha-L-arabinopyranosyl-(1->6)-beta-D-glucopyranose, activity is lower than hydrolysis of the 3-O-beta-D-glucopyranosyl-(1->2)-beta-D-glucopyranosyl linkage in panaxadiol Sulfolobus acidocaldarius ginsenoside K + L-arabinopyranose
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 ?