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Literature summary for 3.2.1.B34 extracted from
- Hydrolysis and transglycosylation activity of a thermostable recombinant beta-glycosidase from Sulfolobus acidocaldarius (2010), Appl. Biochem. Biotechnol., 160, 2236-2247.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Sulfolobus acidocaldarius |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.116 | - |
2-nitrophenyl beta-D-fucopyranoside | pH 5.5, 90°C | Sulfolobus acidocaldarius |  |
| 0.138 | - |
2-nitrophenyl beta-D-glucopyranoside | pH 5.5, 90°C | Sulfolobus acidocaldarius | |
| 0.139 | - |
4-nitrophenyl beta-D-fucopyranoside | pH 5.5, 90°C | Sulfolobus acidocaldarius | |
| 0.318 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.5, 90°C | Sulfolobus acidocaldarius | |
| 1.45 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 5.5, 90°C | Sulfolobus acidocaldarius | |
| 3.18 | - |
2-nitrophenyl beta-D-galactopyranoside | pH 5.5, 90°C | Sulfolobus acidocaldarius | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57000 | - |
2 * 57000, SDS-PAGE | Sulfolobus acidocaldarius |
| 57143 | - |
2 * 57143, calculated from sequence | Sulfolobus acidocaldarius |
| 114000 | - |
gel filtration | Sulfolobus acidocaldarius |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sulfolobus acidocaldarius | P14288 | - |
- |
| Sulfolobus acidocaldarius DSM 639 | P14288 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sulfolobus acidocaldarius |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 58 | - |
pH 5.5, 90°C | Sulfolobus acidocaldarius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-nitrophenyl beta-D-fucopyranoside + H2O | - |
Sulfolobus acidocaldarius | 2-nitrophenol + D-fucopyranose | - |
? | |
| 2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Sulfolobus acidocaldarius | 2-nitrophenol + D-galactopyranose | - |
? | |
| 2-nitrophenyl beta-D-glucopyranoside + H2O | - |
Sulfolobus acidocaldarius | 2-nitrophenol + D-glucopyranose | - |
? | |
| 2-nitrophenyl beta-D-glucopyranoside + H2O | - |
Sulfolobus acidocaldarius DSM 639 | 2-nitrophenol + D-glucopyranose | - |
? | |
| 4-nitrophenyl beta-D-fucopyranoside + H2O | hydrolytic activity with 4-nitrophenyl substrates in the order of decreasing efficiency: 4-nitrophenyl beta-D-fucopyranoside, 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl beta-D-mannopyranoside, 4-nitrophenyl beta-D-xylopyranoside | Sulfolobus acidocaldarius | 4-nitrophenol + D-fucopyranose | - |
? | |
| 4-nitrophenyl beta-D-galactopyranoside + H2O | hydrolytic activity with 4-nitrophenyl substrates in the order of decreasing efficiency: 4-nitrophenyl beta-D-fucopyranoside, 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl beta-D-mannopyranoside, 4-nitrophenyl beta-D-xylopyranoside | Sulfolobus acidocaldarius | 4-nitrophenol + D-galactopyranose | - |
? | |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | hydrolytic activity with 4-nitrophenyl substrates in the order of decreasing efficiency: 4-nitrophenyl beta-D-fucopyranoside, 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl beta-D-mannopyranoside, 4-nitrophenyl beta-D-xylopyranoside | Sulfolobus acidocaldarius | 4-nitrophenol + D-glucopyranose | - |
? | |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | hydrolytic activity with 4-nitrophenyl substrates in the order of decreasing efficiency: 4-nitrophenyl beta-D-fucopyranoside, 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl beta-D-mannopyranoside, 4-nitrophenyl beta-D-xylopyranoside | Sulfolobus acidocaldarius DSM 639 | 4-nitrophenol + D-glucopyranose | - |
? | |
| 4-nitrophenyl beta-D-mannopyranoside + H2O | hydrolytic activity with 4-nitrophenyl substrates in the order of decreasing efficiency: 4-nitrophenyl beta-D-fucopyranoside, 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl beta-D-mannopyranoside, 4-nitrophenyl beta-D-xylopyranoside | Sulfolobus acidocaldarius | 4-nitrophenol + D-mannopyranose | - |
? | |
| 4-nitrophenyl beta-D-xylopyranoside + H2O | hydrolytic activity with 4-nitrophenyl substrates in the order of decreasing efficiency: 4-nitrophenyl beta-D-fucopyranoside, 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl beta-D-mannopyranoside, 4-nitrophenyl beta-D-xylopyranoside | Sulfolobus acidocaldarius | 4-nitrophenol + D-xylopyranose | - |
? | |
| cellobiose + H2O | - |
Sulfolobus acidocaldarius | 2 D-glucose | - |
? | |
| cellobiose + H2O | - |
Sulfolobus acidocaldarius DSM 639 | 2 D-glucose | - |
? | |
| lactose + H2O | - |
Sulfolobus acidocaldarius | D-galactose + D-glucose | - |
? | |
| lactose + H2O | - |
Sulfolobus acidocaldarius DSM 639 | D-galactose + D-glucose | - |
? | |
| additional information | no activity toward aryl-alpha-glycosides or 4-nitrophenyl beta-L-arabinofuranoside. The enzyme exhibits transglycosylation activity with 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl beta-D-glucopyranoside, and 4-nitrophenyl beta-D-fucopyranoside in decreasing order of activity, in the reverse order of its hydrolytic activity. The hydrolytic activity is higher toward cellobiose than toward lactose, but the transglycosylation activity is lower with cellobiose than with lactose | Sulfolobus acidocaldarius | ? | - |
? | |
| additional information | no activity toward aryl-alpha-glycosides or 4-nitrophenyl beta-L-arabinofuranoside. The enzyme exhibits transglycosylation activity with 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl beta-D-glucopyranoside, and 4-nitrophenyl beta-D-fucopyranoside in decreasing order of activity, in the reverse order of its hydrolytic activity. The hydrolytic activity is higher toward cellobiose than toward lactose, but the transglycosylation activity is lower with cellobiose than with lactose | Sulfolobus acidocaldarius DSM 639 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 57000, SDS-PAGE | Sulfolobus acidocaldarius |
| dimer | 2 * 57143, calculated from sequence | Sulfolobus acidocaldarius |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BgaS | - |
Sulfolobus acidocaldarius |
| Saci_1849 | locus name | Sulfolobus acidocaldarius |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
- |
Sulfolobus acidocaldarius |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | 100 | 80°C: about 70% of maximal activity, 100°C: about 40% of maximal activity | Sulfolobus acidocaldarius |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
half-lfe: 494 h | Sulfolobus acidocaldarius |
| 80 | - |
half-lfe: 60 h | Sulfolobus acidocaldarius |
| 90 | - |
half-lfe: 0.2 h | Sulfolobus acidocaldarius |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 9.01 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 5.5, 90°C | Sulfolobus acidocaldarius | |
| 14.6 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.5, 90°C | Sulfolobus acidocaldarius | |
| 16.7 | - |
2-nitrophenyl beta-D-glucopyranoside | pH 5.5, 90°C | Sulfolobus acidocaldarius | |
| 18.6 | - |
4-nitrophenyl beta-D-fucopyranoside | pH 5.5, 90°C | Sulfolobus acidocaldarius | |
| 26.4 | - |
2-nitrophenyl beta-D-fucopyranoside | pH 5.5, 90°C | Sulfolobus acidocaldarius | |
| 31.5 | - |
2-nitrophenyl beta-D-galactopyranoside | pH 5.5, 90°C | Sulfolobus acidocaldarius | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Sulfolobus acidocaldarius |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6.5 | pH 5.5: about 80% of maximal activity, pH 6.5: about 60% of maximal activity | Sulfolobus acidocaldarius |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6.2 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 5.5, 90°C | Sulfolobus acidocaldarius | |
| 9.9 | - |
2-nitrophenyl beta-D-galactopyranoside | pH 5.5, 90°C | Sulfolobus acidocaldarius | |
| 46 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.5, 90°C | Sulfolobus acidocaldarius | |
| 121 | - |
2-nitrophenyl beta-D-glucopyranoside | pH 5.5, 90°C | Sulfolobus acidocaldarius | |
| 134 | - |
4-nitrophenyl beta-D-fucopyranoside | pH 5.5, 90°C | Sulfolobus acidocaldarius | |
| 228 | - |
2-nitrophenyl beta-D-fucopyranoside | pH 5.5, 90°C | Sulfolobus acidocaldarius | |
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