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Literature summary for 3.2.1.B34 extracted from

  • Petzelbauer, I.; Reiter, A.; Splechtna, B.; Kosma, P.; Nidetzky, B.
    Transgalactosylation by thermostable beta-glycosidases from Pyrococcus furiosus and Sulfolobus solfataricus. Binding interactions of nucleophiles with the galactosylated enzyme intermediate make major contributions to the formation of new beta-glycosides (2000), Eur. J. Biochem., 267, 5055-5066.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1
-
2-nitrophenyl beta-D-galactoside release of 2-nitrophenol, pH 7.5, 80°C Saccharolobus solfataricus
192
-
methyl beta-D-galactoside relase, of methanol, pH 7.5, 80°C Saccharolobus solfataricus
196
-
lactose release of D-glucose, pH 7.5, 80°C Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus P22498
-
-
Saccharolobus solfataricus DSM 1617 P22498
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-galactoside
-
Saccharolobus solfataricus 2-nitrophenol + beta-D-galactose
-
r
lactose + H2O
-
Saccharolobus solfataricus D-glucose + D-galactose
-
r
methyl beta-D-galactoside
-
Saccharolobus solfataricus methanol + beta-D-galactose
-
r
additional information enzyme additionally catalyzes transglycosylation reactions, making new beta(1->3) and beta(1->6) glycosidic bonds by intermolecular as well as intramolecular transfer reactions. The intramolecular galactosyl transfer of CelB yields beta-D-Galp-(1->6)-D-glucose and beta-D-Galp-(1->3)-D-glucose in a molar ratio of about 1 : 2. Galactosyl transfer from CelB to D-glucose occurs with partitioning ratios, kNu /kwater, which are 170 times those for the reactions of the galactosylated enzyme with 2-propanol. Therefore, the binding interactions with nucleophiles contribute chiefly to formation of new beta-glycosides during lactose conversion. Likewise, noncovalent interactions with the glucose leaving group govern the catalytic efficiencies for the hydrolysis of lactose Saccharolobus solfataricus ?
-
?
additional information enzyme additionally catalyzes transglycosylation reactions, making new beta(1->3) and beta(1->6) glycosidic bonds by intermolecular as well as intramolecular transfer reactions. The intramolecular galactosyl transfer of CelB yields beta-D-Galp-(1->6)-D-glucose and beta-D-Galp-(1->3)-D-glucose in a molar ratio of about 1 : 2. Galactosyl transfer from CelB to D-glucose occurs with partitioning ratios, kNu /kwater, which are 170 times those for the reactions of the galactosylated enzyme with 2-propanol. Therefore, the binding interactions with nucleophiles contribute chiefly to formation of new beta-glycosides during lactose conversion. Likewise, noncovalent interactions with the glucose leaving group govern the catalytic efficiencies for the hydrolysis of lactose Saccharolobus solfataricus DSM 1617 ?
-
?

Synonyms

Synonyms Comment Organism
LACS
-
Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.6
-
methyl beta-D-galactoside relase, of methanol, pH 7.5, 80°C Saccharolobus solfataricus
1300
-
2-nitrophenyl beta-D-galactoside release of 2-nitrophenol, pH 7.5, 80°C Saccharolobus solfataricus
1500
-
lactose release of D-glucose, pH 7.5, 80°C Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.034
-
methyl beta-D-galactoside relase, of methanol, pH 7.5, 80°C Saccharolobus solfataricus
7.7
-
lactose release of D-glucose, pH 7.5, 80°C Saccharolobus solfataricus
1200
-
2-nitrophenyl beta-D-galactoside release of 2-nitrophenol, pH 7.5, 80°C Saccharolobus solfataricus