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Literature summary for 3.2.1.B33 extracted from
- Production of a new sucrose derivative by transglycosylation of recombinant Sulfolobus shibatae beta-glycosidase (2005), Carbohydr. Res., 340, 1089-1096.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Saccharolobus shibatae |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57000 | - |
x * 57000, SDS-PAGE | Saccharolobus shibatae |
| 57400 | - |
MALDI-TOF MS | Saccharolobus shibatae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus shibatae | P50388 | - |
- |
| Saccharolobus shibatae JCM 8931 | P50388 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharolobus shibatae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 292.5 | - |
75°C, pH not specified in the publication | Saccharolobus shibatae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Saccharolobus shibatae | 4-nitrophenol + D-glucopyranose | - |
? |  |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Saccharolobus shibatae JCM 8931 | 4-nitrophenol + D-glucopyranose | - |
? | |
| additional information | transglycosylation activity is tested with lactose as a donor and various sugars as acceptors. When sucrose is used as an acceptor beta-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-beta-D-fructofuranoside is formed | Saccharolobus shibatae | ? | - |
? | |
| additional information | transglycosylation activity is tested with lactose as a donor and various sugars as acceptors. When sucrose is used as an acceptor beta-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-beta-D-fructofuranoside is formed | Saccharolobus shibatae JCM 8931 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 57000, SDS-PAGE | Saccharolobus shibatae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
assay at | Saccharolobus shibatae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
assay at | Saccharolobus shibatae |
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Release 2023.1 (January 2023)
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