Literature summary for 3.2.1.B28 extracted from

Zeuner, B.; Nyffenegger, C.; Mikkelsen, J.D.; Meyer, A.S.
Thermostable beta-galactosidases for the synthesis of human milk oligosaccharides (2016), N. Biotechnol., 33, 355-60.

Search for more literature for 3.2.1.B28

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Pyrococcus furiosus

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus furiosus	Q51723	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
lactose + N,N'-diacetylchitobiose	-	Pyrococcus furiosus	Gal-beta(1,4)-GlcNAc-beta(1,3)-Gal-beta(1,4)-Glc + H2O	-	?
additional information	enzyme is able to catalyze formation of lacto-N-neotetraose, i.e. Gal-beta(1,4)-GlcNAc-beta(1,3)-Gal-beta(1,4)-Glc, from lactose and N,N'-diacetylchitobiose and of N-acetyllactosamine, i.e Gal-beta(1,4)-GlcNAc from N-acetylglucosamine and lactose. When compared with beta-glycosidase BglT from Thermus thermophilus, and beta-galactosidase BgaD-D from Bacillus circulans, BgaD is the most potent transgalactosidase, but both BglT and CelB can catalyze formation of LNnT and LacNAc, with BglT giving higher yields than CelB	Pyrococcus furiosus	?	-	?
N-acetyl-D-glucosamine + lactose	-	Pyrococcus furiosus	4-O-beta-D-galactopyranosyl-N-acetyl-D-glucosamine + D-glucose	transgalactosylation reaction	?

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
80	-	half-life 280 min	Pyrococcus furiosus
90	-	half-life 205 min	Pyrococcus furiosus
95	-	half-life 11 min	Pyrococcus furiosus

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Release 2023.1 (January 2023)