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Literature summary for 3.2.1.B28 extracted from
- Production of aglycon protopanaxadiol via compound K by a thermostable beta-glycosidase from Pyrococcus furiosus (2011), Appl. Microbiol. Biotechnol., 89, 1019-1028.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | beta-glycosidase converts ginsenosides Rb1, Rb2, Rc, and Rd to protopanaxadiol aglycone via compound K. With increases in the enzyme activity, the productivities increase. The substrate concentration is optimal at ginsenoside Rd or 10% (w/v) ginseng root extract. 4 mM of ginsenoside Rd is converted to 3.3 mM compound K with a yield of 82.5% (mol/mol) and a productivity of 2010 mg per l and h at 1 h and is hydrolyzed completely to the aglycone with 364 mg per l and h after 5 h | Pyrococcus furiosus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55488 | - |
x * 55500, SDS-PAGE, x * 55488, calculated | Pyrococcus furiosus |
| 55500 | - |
x * 55500, SDS-PAGE, x * 55488, calculated | Pyrococcus furiosus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus furiosus | Q51723 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.08 | - |
substrate ginsenoside Rc, pH 5.5, 95°C | Pyrococcus furiosus |
| 0.55 | - |
substrate ginsenoside Rb2, pH 5.5, 95°C | Pyrococcus furiosus |
| 4.5 | - |
substrate 4-nitrophenyl alpha-L-arabinofuranoside, pH 5.5, 95°C | Pyrococcus furiosus |
| 4.5 | - |
substrate ginsenoside Rb1, pH 5.5, 95°C | Pyrococcus furiosus |
| 5.5 | - |
substrate ginsenoside Rd, pH 5.5, 95°C | Pyrococcus furiosus |
| 15 | - |
substrate 4-nitrophenyl alpha-L-arabinopyranoside, pH 5.5, 95°C | Pyrococcus furiosus |
| 529 | - |
substrate 2-nitrophenyl beta-D-galactopyranoside, pH 5.5, 95°C | Pyrococcus furiosus |
| 561 | - |
substrate 4-nitrophenyl beta-D-galactopyranoside, pH 5.5, 95°C | Pyrococcus furiosus |
| 828 | - |
substrate 2-nitrophenyl beta-D-glucopyranoside, pH 5.5, 95°C | Pyrococcus furiosus |
| 912 | - |
substrate 4-nitrophenyl beta-D-glucopyranoside, pH 5.5, 95°C | Pyrococcus furiosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Pyrococcus furiosus | 2-nitrophenol + D-galactose | - |
? |  |
| 2-nitrophenyl beta-D-glucopyranoside + H2O | - |
Pyrococcus furiosus | 2-nitrophenol + D-glucose | - |
? | |
| 4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Pyrococcus furiosus | 4-nitrophenol + L-arabinose | - |
? | |
| 4-nitrophenyl alpha-L-arabinopyranoside + H2O | - |
Pyrococcus furiosus | 4-nitrophenol + L-arabinose | - |
? | |
| 4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Pyrococcus furiosus | 4-nitrophenol + D-galactose | - |
? | |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Pyrococcus furiosus | 4-nitrophenol + D-glucose | - |
? | |
| ginsenoside Rb1 + 4 H2O | - |
Pyrococcus furiosus | protopanaxadiol aglycone + 4 D-glucopyranose | - |
? | |
| ginsenoside Rb2 + 4 H2O | - |
Pyrococcus furiosus | protopanaxadiol aglycone + 3 D-glucopyranose + L-arabinopyranose | - |
? | |
| ginsenoside Rc + 4 H2O | - |
Pyrococcus furiosus | protopanaxadiol aglycone + 3 D-glucopyranose + L-arabinopyranose | - |
? | |
| ginsenoside Rd + 3 H2O | - |
Pyrococcus furiosus | protopanaxadiol aglycone + 3 D-glucopyranose | - |
? | |
| additional information | enzyme converts ginsenosides Rb1, Rb2, Rc, and Rd to protopanaxadiol aglycone via compound K | Pyrococcus furiosus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55500, SDS-PAGE, x * 55488, calculated | Pyrococcus furiosus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CelB | - |
Pyrococcus furiosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 95 | - |
- |
Pyrococcus furiosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 95 | - |
half-life 68 h | Pyrococcus furiosus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Pyrococcus furiosus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6 | more than 80% of maximum activity | Pyrococcus furiosus |
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