Literature summary for 3.2.1.B28 extracted from

Splechtna, B.; Petzelbauer, I.; Kuhn, B.; Kulbe, K.D.; Nidetzky, B.
Hydrolysis of lactose by beta-glycosidase CelB from hyperthermophilic archaeon Pyrococcus furiosus: comparison of hollow-fiber membrane and packed-bed immobilized enzyme reactors for continuous processing of ultrahigh temperature-treated skim milk (2002), Appl. Biochem. Biotechnol., 98-100, 473-88.

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Application

Application	Comment	Organism
synthesis	expression of CleB gene in Escherichia coli gives approx 100000 U of enzyme activity/l of culture medium after 8 h of growth	Pyrococcus furiosus
synthesis	hydrolysis of lactose in UHT skim milk at 70°C using CelB covalently attached onto Eupergit C in yields of 80%, and in a packed-bed immobilized enzyme reactor. The packed-bed reactor is about 10fold more stable and gives about the same productivity at 80% substrate conversion as the hollow-fiber reactor at 60% substrate conversion. The marked difference in the stability of free and immobilized CelB seems to reflect mainly binding of the soluble enzyme to the membrane surface of the hollow-fiber module. Microbial contamination of the reactors did not occur during reaction times of up to 39 d	Pyrococcus furiosus

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Pyrococcus furiosus

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus furiosus	Q51723	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
lactose + H2O	-	Pyrococcus furiosus	D-glucose + D-galactose	-	?

Synonyms

Synonyms	Comment	Organism
CelB	-	Pyrococcus furiosus

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Release 2023.1 (January 2023)