Cloned (Comment) | Organism |
---|---|
recombinant mutant that lacks the C-terminal 23 residues and includes six substitutive mutations R170A, R220A, Y227F, F447S, R448V and E449Kis expressed in Escherichia coli BL21 | Pyrococcus furiosus |
Crystallization (Comment) | Organism |
---|---|
hanging-drop vapour-diffusion method, a monomeric form of the enzyme is constructed by removing the C-terminal region (the mutant lacks the C-terminal 23 residues and includes six substitutive mutations R170A, R220A, Y227F, F447S, R448V and E449K) of the enzyme and its crystal structure is solved at a resolution of 2.8 A in space group | Pyrococcus furiosus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | a mutant lacks the C-terminal 23 residues and includes six substitutive mutations R170A, R220A, Y227F, F447S, R448V and E449K forms a unique dodecameric structure consisting of two hexameric rings in the asymmetric unit of the crystal. The lack of the C-terminal region does not affect the activity of the enzyme, but disrupts its oligomeric state and hyperthermostability | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | Q51723 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
Subunits | Comment | Organism |
---|---|---|
dodecamer | a monomeric form of the enzyme is constructed by removing the C-terminal region of the enzyme (the mutant lacks the C-terminal 23 residues and includes six substitutive mutations R170A, R220A, Y227F, F447S, R448V and E449K). The mutant enzyme forms a unique dodecameric structure consisting of two hexameric rings in the asymmetric unit of the crystal | Pyrococcus furiosus |
monomer | a monomeric form of the enzyme is constructed by removing the C-terminal region of the enzyme (the mutant lacks the C-terminal 23 residues and includes six substitutive mutations R170A, R220A, Y227F, F447S, R448V and E449K). The mutant enzyme forms a unique dodecameric structure consisting of two hexameric rings in the asymmetric unit of the crystal | Pyrococcus furiosus |
tetramer | the native enzyme forms a stable tetrameric structure | Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
BGLPf | - |
Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | 80 | the mutant enzyme that lacks the C-terminal 23 residues and includes six substitutive mutations R170A, R220A, Y227F, F447S, R448V and E449K is immediately inactivated between 75 and 80°C | Pyrococcus furiosus |
85 | - |
wild-type enzyme is stable beyond 85°C | Pyrococcus furiosus |
110 | - |
Tm-value of wild-type enzyme | Pyrococcus furiosus |