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Literature summary for 3.2.1.B28 extracted from

  • Lang, M.; Kamrat, T.; Nidetzky, B.
    Influence of ionic liquid cosolvent on transgalactosylation reactions catalyzed by thermostable beta-glycosylhydrolase CelB from Pyrococcus furiosus (2006), Biotechnol. Bioeng., 95, 1093-1100.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1,3-dimethylimidazolium methylsulfate the enzyme retains full catalytic efficiency for lactose hydrolysis at 80°C in a 50% (v/v) solution of the ionic liquid 1,3-dimethylimidazoliummethylsulfate in sodium citrate buffer, pH 5.5. It is inactive but not irreversibly denatured at 70% (v/v) ionic liquid Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q51723
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the transferase activity of the enzyme can be improved significantly by addition of the ionic liquid 1,3-dimethylimidazoliummethylsulfate. The relative enhancement of the selectivity of the enzyme for glycosyl transfer to the acceptor nucleophile compared with water by the cosolvent is strongly dependent on the structure of the acceptor, suggesting that interactions of the acceptor with solvent molecules, water or ionic liquid, make a major contribution to the observed transgalactosylation specificity of the enzyme Pyrococcus furiosus ?
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Synonyms

Synonyms Comment Organism
CelB
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Pyrococcus furiosus