Literature summary for 3.2.1.B28 extracted from

B�hmer, N.; Lutz-Wahl, S.; Fischer, L.
Recombinant production of hyperthermostable CelB from Pyrococcus furiosus in Lactobacillus sp. (2012), Appl. Microbiol. Biotechnol., 96, 903-912.

Search for more literature for 3.2.1.B28

Cloned(Commentary)

Cloned (Comment)	Organism
expression in in Lactobacillus plantarum NC8 and Lactobacillus casei as hosts. Both lactobacilli harboring the pSIP409-celB vector produce active CelB in batch bioreactor cultivations while the specific CelB activity of the cell free extract is about 44% higher with Lactobacillus plantarum than with Lactobacillus casei using 4-nitrophenyl beta-galactoside as the substrate. A fedbatch bioreactor cultivation of Lactobacillus plantarum NC8 pSIP409-celB results in a specific CelB activity of 2500 nkat 4-nitrophenyl beta-D-galactopyranoside/mg protein after 28 h. A repeated dosage of the inducer (sakacin P inducing peptide) does not increase the enzyme expression further	Pyrococcus furiosus

Cloned (Comment)

Organism

expression in in Lactobacillus plantarum NC8 and Lactobacillus casei as hosts. Both lactobacilli harboring the pSIP409-celB vector produce active CelB in batch bioreactor cultivations while the specific CelB activity of the cell free extract is about 44% higher with Lactobacillus plantarum than with Lactobacillus casei using 4-nitrophenyl beta-galactoside as the substrate. A fedbatch bioreactor cultivation of Lactobacillus plantarum NC8 pSIP409-celB results in a specific CelB activity of 2500 nkat 4-nitrophenyl beta-D-galactopyranoside/mg protein after 28 h. A repeated dosage of the inducer (sakacin P inducing peptide) does not increase the enzyme expression further

Pyrococcus furiosus

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus furiosus	Q51723	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl beta-D-galactopyranoside + H2O	-	Pyrococcus furiosus	4-nitrophenol + beta-D-galactopyranose	-	?

Synonyms

Synonyms	Comment	Organism
CelB	-	Pyrococcus furiosus

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Release 2023.1 (January 2023)