Literature summary for 3.2.1.B26 extracted from

Catanzano, F.; Graziano, G.; De Paola, B.; Barone, G.; D'Auria, S.; Rossi, M.; Nucci, R.
Guanidine-induced denaturation of beta-glycosidase from Sulfolobus solfataricus expressed in Escherichia coli (1998), Biochemistry, 37, 14484-14490.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Saccharolobus solfataricus

Organic Solvent Stability

Organic Solvent	Comment	Organism
guanidine-HCl	guanidine-induced denaturation is reversible when the protein concentration is lower than 0.01 mg/ml. In the range 2-4 M guanidine-HCl, there is an equilibrium among tetrameric, dimeric, and monomeric species. These findings indicate that the guanidine-induced denaturation is not a two-state transition with concomitant unfolding and dissociation of the four subunits. A mechanism involving a dimeric intermediate species is proposed and is able to fit the experimental fluorescence intensity transition profiles, allowing the estimation of the total denaturation Gibbs energy change at 25°C and pH 6.5	Saccharolobus solfataricus

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	-	-	-

Renatured (Commentary)

Renatured (Comment)	Organism
guanidine-induced denaturation is reversible when the protein concentration is lower than 0.01 mg/ml. In the range 2-4 M guanidine-HCl, there is an equilibrium among tetrameric, dimeric, and monomeric species. These findings indicate that the guanidine-induced denaturation is not a two-state transition with concomitant unfolding and dissociation of the four subunits. A mechanism involving a dimeric intermediate species is proposed and is able to fit the experimental fluorescence intensity transition profiles, allowing the estimation of the total denaturation Gibbs energy change at 25°C and pH 6.5	Saccharolobus solfataricus

Renatured (Comment)

Organism

guanidine-induced denaturation is reversible when the protein concentration is lower than 0.01 mg/ml. In the range 2-4 M guanidine-HCl, there is an equilibrium among tetrameric, dimeric, and monomeric species. These findings indicate that the guanidine-induced denaturation is not a two-state transition with concomitant unfolding and dissociation of the four subunits. A mechanism involving a dimeric intermediate species is proposed and is able to fit the experimental fluorescence intensity transition profiles, allowing the estimation of the total denaturation Gibbs energy change at 25°C and pH 6.5

Saccharolobus solfataricus

Synonyms

Synonyms	Comment	Organism
Sbetagly	-	Saccharolobus solfataricus