Protein Variants | Comment | Organism |
---|---|---|
E206Q | mutant shows 10- and 60-fold reduced activities on aryl-galacto and aryl-glucosides, respectively, when compared with the wild type. Significant decrease in Km-value with 4-nitrophenyl beta-D-glucoside or 2-nitrophenyl beta-D-glucoside. The residual activity of the mutant loses the typical pH dependence shown by the wild type. These data suggest that Glu206 acts as the general acid/base catalyst in the hydrolysis reaction | Saccharolobus solfataricus |
E206Q | mutant shows 10- and 60-fold reduced activities on aryl-galacto and aryl-glucosides, respectively, when compared with the wild type. Significant decrease in Km-value with 4-nitrophenyl beta-D-glucoside or 2-nitrophenyl betaD-glucoside. The residual activity of the mutant loses the typical pH dependence shown by the wild type. These data suggest that Glu206 acts as the general acid/base catalyst in the hydrolysis reaction | Saccharolobus solfataricus |
E387Q | mutant shows no detectable activity | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
4-nitrophenyl beta-D-glucoside | pH 6.5, 65°C, mutant enzyme E206Q | Saccharolobus solfataricus | |
0.03 | - |
2-Nitrophenyl beta-D-glucoside | pH 6.5, 65°C, mutant enzyme E206Q | Saccharolobus solfataricus | |
0.09 | - |
4-nitrophenyl beta-D-fucoside | pH 6.5, 65°C, mutant enzyme E206Q | Saccharolobus solfataricus | |
0.3 | - |
4-nitrophenyl beta-D-glucoside | pH 6.5, 65°C, wild-type enzyme | Saccharolobus solfataricus | |
0.45 | - |
4-nitrophenyl beta-D-fucoside | pH 6.5, 65°C, wild-type enzyme | Saccharolobus solfataricus | |
0.95 | - |
2-nitrophenyl beta-D-galactopyranoside | pH 6.5, 65°C, wild-type enzyme | Saccharolobus solfataricus | |
1.01 | - |
2-Nitrophenyl beta-D-glucoside | pH 6.5, 65°C, wild-type enzyme | Saccharolobus solfataricus | |
1.17 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 6.5, 65°C, wild-type enzyme | Saccharolobus solfataricus | |
1.53 | - |
2-nitrophenyl beta-D-galactopyranoside | pH 6.5, 65°C, mutant enzyme E206Q | Saccharolobus solfataricus | |
4.79 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 6.5, 65°C, mutant enzyme E206Q | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Saccharolobus solfataricus MT4 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purification of mutant proteins E206Q and E387Q to homogeneity using the Schistosoma japonicum glutathione S-transferase fusion system | Saccharolobus solfataricus |
purification of mutant proteins E206Q and E387Q to homogeneity using the Schistosoma japonicum glutathione-S-transferase fusion system | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Saccharolobus solfataricus | 2-nitrophenol + beta-D-galactopyranose | - |
? | |
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Saccharolobus solfataricus MT4 | 2-nitrophenol + beta-D-galactopyranose | - |
? | |
2-nitrophenyl beta-D-glucoside + H2O | - |
Saccharolobus solfataricus | 2-nitrophenol + beta-D-glucose | - |
? | |
2-nitrophenyl beta-D-glucoside + H2O | - |
Saccharolobus solfataricus MT4 | 2-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-fucoside + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + beta-D-fucose | - |
? | |
4-nitrophenyl beta-D-fucoside + H2O | - |
Saccharolobus solfataricus MT4 | 4-nitrophenol + beta-D-fucose | - |
? | |
4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + beta-D-galactopyranose | - |
? | |
4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Saccharolobus solfataricus MT4 | 4-nitrophenol + beta-D-galactopyranose | - |
? | |
4-nitrophenyl beta-D-glucoside + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucoside + H2O | - |
Saccharolobus solfataricus MT4 | 4-nitrophenol + beta-D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Sbetagly | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
assay at | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.4 | - |
4-nitrophenyl beta-D-glucoside | pH 6.5, 65°C, mutant enzyme E206Q | Saccharolobus solfataricus | |
4.5 | - |
2-Nitrophenyl beta-D-glucoside | pH 6.5, 65°C, mutant enzyme E206Q | Saccharolobus solfataricus | |
31 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 6.5, 65°C, mutant enzyme E206Q | Saccharolobus solfataricus | |
34 | - |
2-nitrophenyl beta-D-galactopyranoside | pH 6.5, 65°C, mutant enzyme E206Q | Saccharolobus solfataricus | |
35 | - |
4-nitrophenyl beta-D-fucoside | pH 6.5, 65°C, mutant enzyme E206Q | Saccharolobus solfataricus | |
240 | - |
4-nitrophenyl beta-D-glucoside | pH 6.5, 65°C, wild-type enzyme | Saccharolobus solfataricus | |
252 | - |
2-Nitrophenyl beta-D-glucoside | pH 6.5, 65°C, wild-type enzyme | Saccharolobus solfataricus | |
263 | - |
4-nitrophenyl beta-D-fucoside | pH 6.5, 65°C, wild-type enzyme | Saccharolobus solfataricus | |
275 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 6.5, 65°C, wild-type enzyme | Saccharolobus solfataricus | |
295 | - |
2-nitrophenyl beta-D-galactopyranoside | pH 6.5, 65°C, wild-type enzyme | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Saccharolobus solfataricus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.7 | 8 | pH 5.6: about 45% of maximal activity, pH 8.0: about 55% of maximal activity | Saccharolobus solfataricus |