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Literature summary for 3.2.1.B26 extracted from

  • D'Auria, S.; Morana, A.; Febbraio, F.; Vaccaro, C.; De Rosa, M.; Nucci, R.
    Functional and structural properties of the homogeneous beta-glycosidase from the extreme thermoacidophilic archaeon Sulfolobus solfataricus expressed in Saccharomyces cerevisiae (1996), Protein Expr. Purif., 7, 299-308.
    View publication on PubMed

Application

Application Comment Organism
analysis reporter genes in molecular biology Saccharolobus solfataricus
nutrition catalyst of lactose hydrolysis in dairy products in the food industry Saccharolobus solfataricus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae Saccharolobus solfataricus
expression in Saccharomyces cerevisiae Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
4-nitrophenyl-cellobioside
-
Saccharolobus solfataricus
0.3
-
4-nitrophenyl cellobioside pH 6.5, 75°C, recombinant enzyme Saccharolobus solfataricus
0.3
-
4-nitrophenyl cellobioside pH 6.5, 75°C, wild-type enzyme Saccharolobus solfataricus
0.5
-
4-nitrophenyl-beta-D-glucopyranoside
-
Saccharolobus solfataricus
0.5
-
4-nitrophenyl beta-D-glucoside pH 6.5, 75°C, recombinant enzyme Saccharolobus solfataricus
0.5
-
4-nitrophenyl beta-D-glucoside pH 6.5, 75°C, wild-type enzyme Saccharolobus solfataricus
0.7
-
4-nitrophenyl-beta-D-fucoside
-
Saccharolobus solfataricus
0.7
-
4-nitrophenyl beta-D-fucoside pH 6.5, 75°C, recombinant enzyme Saccharolobus solfataricus
1
-
4-nitrophenyl beta-D-fucoside pH 6.5, 75°C, wild-type enzyme Saccharolobus solfataricus
1.4
-
laminaribiose
-
Saccharolobus solfataricus
3.9
-
2-nitrophenyl-beta-D-galactopyranoside
-
Saccharolobus solfataricus
3.9
-
2-nitrophenyl beta-D-galactopyranoside pH 6.5, 75°C, recombinant enzyme Saccharolobus solfataricus
4
-
2-nitrophenyl beta-D-galactopyranoside pH 6.5, 75°C, wild-type enzyme Saccharolobus solfataricus
4.3
-
4-nitrophenyl-beta-D-galactopyranoside
-
Saccharolobus solfataricus
4.3
-
4-nitrophenyl beta-D-galactopyranoside pH 6.5, 75°C, recombinant enzyme Saccharolobus solfataricus
4.7
-
4-nitrophenyl beta-D-galactopyranoside pH 6.5, 75°C, wild-type enzyme Saccharolobus solfataricus
31.5
-
cellobiose
-
Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
4 * 60000, SDS-PAGE Saccharolobus solfataricus
240000
-
gel filtration Saccharolobus solfataricus
240000
-
gel filtration, glycerol gradient centrifugation Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Saccharolobus solfataricus laminaribiose beta-D-glucose + D-glucose
-
?

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharolobus solfataricus
to homogeneity, recombinant enzyme Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
166
-
-
Saccharolobus solfataricus
166
-
pH 6.5, 75°C Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-galactopyranoside + H2O
-
Saccharolobus solfataricus 2-nitrophenol + beta-D-galactopyranose
-
?
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O laminaribiose Saccharolobus solfataricus beta-D-glucose + D-glucose
-
?
4-nitrophenyl beta-cellobioside + 2 H2O
-
Saccharolobus solfataricus 4-nitrophenol + 2 beta-D-glucose
-
?
4-nitrophenyl beta-D-fucoside + H2O
-
Saccharolobus solfataricus 4-nitrophenol + beta-D-fucose
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
-
Saccharolobus solfataricus 4-nitrophenol + beta-D-galactopyranose
-
?
4-nitrophenyl beta-D-galactoside + H2O
-
Saccharolobus solfataricus 4-nitrophenol + beta-D-galactose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Saccharolobus solfataricus 4-nitrophenol + D-glucopyranose
-
?
4-nitrophenyl beta-D-glucoside + H2O
-
Saccharolobus solfataricus 4-nitrophenol + beta-D-glucose
-
?
4-nitrophenyl cellobioside + H2O
-
Saccharolobus solfataricus 4-nitrophenol + cellobiose
-
?
additional information hydrolase with broad specificity and noticeable glucan 1 -> 4-beta-D-glucosidase activity Saccharolobus solfataricus ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 60000, SDS-PAGE Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
Sbetagly
-
Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
steady increase in activity up to 80°C Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
75
-
5 h, 17% loss of activity Saccharolobus solfataricus
85
-
83% residual activity after 5 h Saccharolobus solfataricus
85
-
5 h, 43% loss of activity Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
448
-
4-nitrophenyl cellobioside pH 6.5, 75°C, recombinant enzyme Saccharolobus solfataricus
503
-
4-nitrophenyl beta-D-fucoside pH 6.5, 75°C, recombinant enzyme Saccharolobus solfataricus
503
-
4-nitrophenyl cellobioside pH 6.5, 75°C, wild-type enzyme Saccharolobus solfataricus
542
-
4-nitrophenyl-beta-D-glucopyranoside
-
Saccharolobus solfataricus
542
-
4-nitrophenyl beta-D-glucoside pH 6.5, 75°C, recombinant enzyme Saccharolobus solfataricus
542
-
4-nitrophenyl beta-D-glucoside pH 6.5, 75°C, wild-type enzyme Saccharolobus solfataricus
772
-
4-nitrophenyl beta-D-fucoside pH 6.5, 75°C, wild-type enzyme Saccharolobus solfataricus
1020
-
4-nitrophenyl beta-D-galactopyranoside pH 6.5, 75°C, wild-type enzyme Saccharolobus solfataricus
1239
-
2-nitrophenyl beta-D-galactopyranoside pH 6.5, 75°C, wild-type enzyme Saccharolobus solfataricus
1285
-
4-nitrophenyl beta-D-galactopyranoside pH 6.5, 75°C, recombinant enzyme Saccharolobus solfataricus
1713
-
2-nitrophenyl beta-D-galactopyranoside pH 6.5, 75°C, recombinant enzyme Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Saccharolobus solfataricus
7
-
in 50 mM sodium phosphate buffer, substrate: 2-nitrophenyl beta-D-galactopyranoside Saccharolobus solfataricus
7
-
in 50 mM sodium phosphate buffer, substrate: 2-nitrophenyl-beta-D-galactopyranoside Saccharolobus solfataricus

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8.5 pH 5.5: about 50% of maximal activity, pH 8.5: about 50% of maximal activity Saccharolobus solfataricus

pI Value

Organism Comment pI Value Maximum pI Value
Saccharolobus solfataricus isoelectric focusing
-
4