Crystallization (Comment) | Organism |
---|---|
hanging-drop vapor-diffusion method using 1.0 mM sodium citrate as a precipitant at pH 6.0. Structure determined at 1.9 A to an R-factor of 18.3% and an R-free-factor of 22.5%. The enzyme molecule has a five-bladed beta-propeller fold. Crystals belong to orthorhombic space group P2(1)2(1)2(1) with unit cell parameters of a = 40.3, b = 77.8 and c = 89.7 A | Geobacillus thermodenitrificans |
Protein Variants | Comment | Organism |
---|---|---|
DELTAV2-W17 | deletion of the N-terminal amino acids causes thermal instability | Geobacillus thermodenitrificans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
x * 33000, mutant enzyme DELTAV2-W17, SDS-PAGE | Geobacillus thermodenitrificans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus thermodenitrificans | - |
- |
- |
Geobacillus thermodenitrificans TS-3 | - |
- |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 33000, mutant enzyme DELTAV2-W17, SDS-PAGE | Geobacillus thermodenitrificans |
Synonyms | Comment | Organism |
---|---|---|
endo-1,5-alpha-L-arabinanase | - |
Geobacillus thermodenitrificans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
mutant enzyme DELTAV2-W17 | Geobacillus thermodenitrificans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
mutant enzyme DELTAV2-W17 loses 65% of its activity after 4 h, half-life: 2.5 h | Geobacillus thermodenitrificans |
65 | - |
mutant enzyme DELTAV2-W17, 70% loss of activity after 5 min | Geobacillus thermodenitrificans |
70 | - |
wild-type enzyme retains more than 80% of its activity after 4 h, complete loss of activity of mutant enzyme DELTAV2-W17 after 5 min | Geobacillus thermodenitrificans |
75 | - |
half-life of wild-type enzyme: 4 h | Geobacillus thermodenitrificans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
mutant enzyme DELTAV2-W17 | Geobacillus thermodenitrificans |