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Literature summary for 3.2.1.98 extracted from
- Role of Trp140 at subsite -6 on the maltohexaose production of maltohexaose-producing amylase from alkalophilic Bacillus sp.707 (2006), Protein Sci., 15, 468-477.
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | production of maltohexaose with low sweetness, low viscosity, and high efficiency for the uptake of energy by humans | Bacillus sp. (in: Bacteria) |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| Wild-type G6-amylase for use in crystallographic analysis produced by the transformants of Bacillus subtilis 207-25, wild-type and mutant G6-amylases for use in activity measurement were produced in a protease-deficient mutant Escherichia coli ME8417 | Bacillus sp. (in: Bacteria) |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging-drop vapor diffusion method | Bacillus sp. (in: Bacteria) |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus sp. (in: Bacteria) | P19571 | - |
- |
| Bacillus sp. (in: Bacteria) 707 | P19571 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| gel filtration | Bacillus sp. (in: Bacteria) |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylose + H2O | - |
Bacillus sp. (in: Bacteria) | maltohexaose | the G6-amylase can hydrolyze almost no maltohexaose and its shorter oligosaccharides as substrates | ? |  |
| amylose + H2O | - |
Bacillus sp. (in: Bacteria) 707 | maltohexaose | the G6-amylase can hydrolyze almost no maltohexaose and its shorter oligosaccharides as substrates | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| G6-amylase | - |
Bacillus sp. (in: Bacteria) |
| Maltohexaose-producing amylase | - |
Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.8 | - |
- |
Bacillus sp. (in: Bacteria) |
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