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Literature summary for 3.2.1.98 extracted from

  • Ben Ali, M.; Khemakhem, B.; Robert, X.; Haser, R.; Bejar, S.
    Thermostability enhancement and change in starch hydrolysis profile of the maltohexaose-forming amylase of Bacillus stearothermophilus US100 strain (2006), Biochem. J., 394, 51-56.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.98

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-
Geobacillus stearothermophilus US100
-
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography using the IMPACT-CN system Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
starch + H2O
-
 Geobacillus stearothermophilus maltohexaose + maltopentaose
-
 ?
starch + H2O
-
 Geobacillus stearothermophilus US100 maltohexaose + maltopentaose
-
 ?

Synonyms

Synonyms Comment Organism
alpha-amylase
-
 Geobacillus stearothermophilus
Amy
-
 Geobacillus stearothermophilus
maltohexaose-forming amylase
-
 Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
 Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
 mutant protein AmyUS100-IG missing Ile214-Gly215 residues, 70 min stable in presence of 25 mM CaCl2 Geobacillus stearothermophilus
100
-
 wild-type Bacillus Amy, 15 min stable in presence of 100 mM CaCl2 Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6
-
-
 Geobacillus stearothermophilus