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Literature summary for 3.2.1.98 extracted from

  • Takasaki, Y.
    Production of maltohexaose by alpha-amylase from Bacillus circulans G-6 (1982), Agric. Biol. Chem., 46, 1539-1547.
No PubMed abstract available
Search for more literature for 3.2.1.98

Inhibitors

Inhibitors Comment Organism Structure
AgNO3 G-6 Niallia circulans
BaCl2 G-6, slight inactivation Niallia circulans
CaCl2 G-6 Niallia circulans
Co2+ G-6, strong inhibition Niallia circulans
CoCl2 G-6, strong inhibition Niallia circulans
Cu2+ G-6, strong inhibition Niallia circulans
CuSO4 G-6, strong inhibition Niallia circulans
Fe2+ G-6, strong inhibition Niallia circulans
FeSO4 G-6, strong inhibition Niallia circulans
Hg2+ G-6, very strong inhibition Niallia circulans
HgCl2 G-6, very strong inhibition Niallia circulans
MnCl2 G-6, slight inactivation Niallia circulans
NiCl2 G-6 Niallia circulans
SrCl2 G-6 Niallia circulans
Zn2+ G-6, strong inhibition Niallia circulans
ZnSO4 G-6, strong inhibition Niallia circulans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Niallia circulans

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Niallia circulans
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
MgSO4 G-6, slight activation Niallia circulans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
76000
-
 G-6, gel filtration Niallia circulans

Organism

Organism UniProt Comment Textmining
Niallia circulans
-
 G-6
-
Niallia circulans G-6
-
 G-6
-

Purification (Commentary)

Purification (Comment) Organism
G-6 Niallia circulans

Reaction

Reaction Comment Organism Reaction ID
(Glcalpha(1-4))m + H2O = (Glcalpha(1-4))m-6 + maltohexaose alpha-amylase, product is linear oligosaccharide containing alpha-1,4-glucosidic linkages Niallia circulans
a

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
 Niallia circulans
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 G-6 Niallia circulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltohexaose + H2O poor substrate Niallia circulans maltose + maltotetraose main product ?
additional information products have alpha-configuration Niallia circulans ?
-
 ?
additional information G-6, alpha-1,6-glucosidic linkages in pullulan, amylopectin etc.: not a substrate Niallia circulans ?
-
 ?
additional information G-6, action pattern Niallia circulans ?
-
 ?
additional information products have alpha-configuration Niallia circulans G-6 ?
-
 ?
additional information G-6, alpha-1,6-glucosidic linkages in pullulan, amylopectin etc.: not a substrate Niallia circulans G-6 ?
-
 ?
additional information G-6, action pattern Niallia circulans G-6 ?
-
 ?
starch + H2O soluble starch Niallia circulans maltohexaose + ? main product ?
starch + H2O soluble starch Niallia circulans maltohexaose + ? G-6, about 30-34% maltohexaose ?
starch + H2O soluble starch Niallia circulans G-6 maltohexaose + ? main product ?
starch + H2O soluble starch Niallia circulans G-6 maltohexaose + ? G-6, about 30-34% maltohexaose ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 G-6 Niallia circulans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 G-6, stable below, Ca2+ not very effective for protecting the enzyme from heat Niallia circulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 G-6 Niallia circulans

pH Range

pH Minimum pH Maximum Comment Organism
5 10 G-6, about 40% of activity at pH 5.0 and 10.0 Niallia circulans

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10 G-6, stable between Niallia circulans