Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.98 extracted from

  • Shirokizawa, O.; Akiba, T.; Horikoshi, K.
    Nucleotide sequence of the G6-amylase gene from alkalophilic Bacillus sp. H-167 (1990), FEMS Microbiol. Lett., 70, 131-135.
    View publication on PubMed
Search for more literature for 3.2.1.98

Cloned(Commentary)

Cloned (Comment) Organism
expressed and multiform G6-amylase produced in Escherichia coli HB101 Bacillus sp. H-167

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 multiple forms G6-amylase having molecular masses: 100000, 90000, 80000, 73000, and 60000, due to intracellular proteolysis of the enzyme Bacillus sp. H-167
100000
-
 multiple forms G6-amylase having molecular masses: 100000, 90000, 80000, 73000, and 60000, due to intracellular proteolysis of the enzyme Bacillus sp. H-167
100000
-
 molecular weight of largest G6-amylase produced in E. coli HB101, SDS-PAGE Bacillus sp. H-167
102600
-
 mature G6-amylase, before proteolytic processing at the COOH-terminal side of enzyme, calculated from nucleotide sequence Bacillus sp. H-167

Organism

Organism UniProt Comment Textmining
Bacillus sp. H-167
-
 alkalophilic bacterium: multiple forms of maltohexaose-forming amylase due to intracellular proteolysis of the enzyme
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus sp. H-167

Synonyms

Synonyms Comment Organism
G6-amylase
-
 Bacillus sp. H-167
maltohexaose-forming amylase
-
 Bacillus sp. H-167

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Bacillus sp. H-167

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.5
-
-
 Bacillus sp. H-167