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Literature summary for 3.2.1.98 extracted from

  • Kainuma, K.; Wako, K.; Kobayashi, S.; Nogami, A.; Suzuki, S.
    Purification and some properties of a novel maltohexaose-producing exo-amylase from Aerobacter aerogenes (1975), Biochim. Biophys. Acta, 410, 333-346.
    View publication on PubMed
Search for more literature for 3.2.1.98

Activating Compound

Activating Compound Comment Organism Structure
cysteine very slight activation Klebsiella pneumoniae

General Stability

General Stability Organism
Ca2+ stabilizes, enzyme is more thermostable in the presence of Ca2+ Klebsiella pneumoniae
substrate has protective effect on enzyme stability Klebsiella pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
AgNO3
-
 Klebsiella pneumoniae
Ba2+ very slight inactivation Klebsiella pneumoniae
Co2+ very slight inactivation Klebsiella pneumoniae
Cu2+
-
 Klebsiella pneumoniae
Hg2+ native enzyme, completely Klebsiella pneumoniae
HgCl2 native enzyme, completely Klebsiella pneumoniae
iodoacetamide slight inactivation Klebsiella pneumoniae
Mg2+ slight inactivation Klebsiella pneumoniae
p-chloromercuribenzoate slight inactivation Klebsiella pneumoniae
Sn2+ slight inactivation Klebsiella pneumoniae
Zn2+
-
 Klebsiella pneumoniae

Localization

Localization Comment Organism GeneOntology No. Textmining
cell bound
-
 Klebsiella pneumoniae
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ native enzyme, activation Klebsiella pneumoniae
Sr2+ activation Klebsiella pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
 gel filtration Klebsiella pneumoniae

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
 Aerobacter aerogenes
-

Purification (Commentary)

Purification (Comment) Organism
-
 Klebsiella pneumoniae

Reaction

Reaction Comment Organism Reaction ID
(Glcalpha(1-4))m + H2O = (Glcalpha(1-4))m-6 + maltohexaose exo-alpha amylase, products have alpha-configuration Klebsiella pneumoniae
a
(Glcalpha(1-4))m + H2O = (Glcalpha(1-4))m-6 + maltohexaose reaction mechanism is exo Klebsiella pneumoniae
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.2
-
 cell-bound enzyme Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O
-
 Klebsiella pneumoniae maltohexaose + ?
-
 ?
amylopectin beta-amylase limit dextrin + H2O
-
 Klebsiella pneumoniae oligosaccharide branched oligosaccharide: B6, B7 and B8 ?
amylose + H2O short-chain amylose, DP, average degree of polymerization, = 23 Klebsiella pneumoniae maltohexaose
-
 ?
amylose + H2O reduced short-chain amylose: about 95% of the rate of short-chain amylose Klebsiella pneumoniae maltohexaose
-
 ?
amylose + H2O high molecular weight amylose: about 50% of the rate of short-chain amylose Klebsiella pneumoniae maltohexaose
-
 ?
glycogen + H2O
-
 Klebsiella pneumoniae maltohexaose
-
 ?
glycogen beta-amylase limit dextrin + H2O poor substrate Klebsiella pneumoniae oligosaccharide branched oligosaccharide: B6, B7 and B8 ?
maltohexaose + H2O poor substrate Klebsiella pneumoniae maltose + maltotetraose main product ?
additional information products have alpha-configuration Klebsiella pneumoniae ?
-
 ?
additional information alpha-cyclodextrin, beta-cyclodextrin, pullulan or maltohexaitol: not a substrate Klebsiella pneumoniae ?
-
 ?
additional information substrate and product specificity, overview Klebsiella pneumoniae ?
-
 ?
additional information exo action pattern Klebsiella pneumoniae ?
-
 ?
starch + H2O
-
 Klebsiella pneumoniae maltohexaose + ? main product ?
starch + H2O
-
 Klebsiella pneumoniae maltohexaose + ? more than 30% maltohexaose ?

Synonyms

Synonyms Comment Organism
maltohexaose producing amylase
-
 Klebsiella pneumoniae
maltohexaose-producing exo-amylase
-
 Klebsiella pneumoniae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 cell-bound enzyme Klebsiella pneumoniae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 80 20°C: about 30% of maximum activity, 80°C: more than 20% of maximum activity Klebsiella pneumoniae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 enzyme more thermostable in the presence of Ca2+ Klebsiella pneumoniae
40
-
 completely stable up to, with Ca2+ Klebsiella pneumoniae
50
-
 15 min at 50°C: 80% of activity retained, with Ca2+ Klebsiella pneumoniae
60
-
 all activity lost after 15 min Klebsiella pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
 cell-bound enzyme Klebsiella pneumoniae

pH Range

pH Minimum pH Maximum Comment Organism
3 9 pH 3.0: 20% of maximum activity, pH 9.0: more than 60% of maximum activity Klebsiella pneumoniae
5.5 8 more than 80% of maximum activity at 5.5 and 8.0 Klebsiella pneumoniae

pH Stability

pH Stability pH Stability Maximum Comment Organism
3
-
 70% loss of activity Klebsiella pneumoniae
6 9 cell-bound enzyme: 20% loss of activity, 60 min, 40°C Klebsiella pneumoniae
10
-
 50% loss of activity Klebsiella pneumoniae