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Literature summary for 3.2.1.98 extracted from
- Enzymatic synthesis of p-nitrophenyl alpha-maltoheptaoside by transglycosylation of maltohexaose-forming amylase (1990), Agric. Biol. Chem., 54, 581-586.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ethanol | transglycosylation, activation | Klebsiella pneumoniae |  |
| methanol | aqueous methanol system, up to 40% methanol, increases transglycosylation | Klebsiella pneumoniae | |
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | enzymatic reaction, transglycosylation, provides practical technique for industrial production of p-nitrophenyl alpha-maltoheptaoside, useful substrate for assay of human alpha-amylase in serum and urine | Klebsiella pneumoniae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-propanol | transglycosylation, inactivation | Klebsiella pneumoniae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | ultraviolet induced mutant | Klebsiella pneumoniae | - |
- |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Ethanol | stable and activation | Klebsiella pneumoniae |
| Methanol | stable up to 40%, at 50% methanol: inactivation of transglycosylation and hydrolysis | Klebsiella pneumoniae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Klebsiella pneumoniae | - |
Aerobacter aerogenes | - |
| Klebsiella pneumoniae | - |
ultraviolet-induced mutant | - |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| (Glcalpha(1-4))m + H2O = (Glcalpha(1-4))m-6 + maltohexaose | mechanism of transglycosylation | Klebsiella pneumoniae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylose + H2O | short-chain amylose, DP, average degree of polymerization, = 23 | Klebsiella pneumoniae | maltohexaose | main product, 40% maltohexaose | ? | |
| maltoheptaose + p-nitrophenyl alpha-glucoside | transglycosylation, mechanism | Klebsiella pneumoniae | p-nitrophenyl alpha-maltoheptaoside + D-glucose | p-nitrophenyl alpha-maltoheptaoside useful substrate for assay of human alpha-amylase in serum and urine | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| G6-forming amylase | - |
Klebsiella pneumoniae |
| maltohexaose-forming amylase | - |
Klebsiella pneumoniae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
transglycosylation | Klebsiella pneumoniae |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
transglycosylation, below, considerable inactivation at | Klebsiella pneumoniae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
transglycosylation, considerabe inactivation | Klebsiella pneumoniae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
transglycosylation | Klebsiella pneumoniae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | transglycosylation | Klebsiella pneumoniae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
transglycosylation, complete inactivation | Klebsiella pneumoniae |
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