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Literature summary for 3.2.1.97 extracted from

  • Gregg, K.J.; Boraston, A.B.
    Cloning, recombinant production, crystallization and preliminary X-ray diffraction analysis of a family 101 glycoside hydrolase from Streptococcus pneumoniae (2009), Acta Crystallogr. Sect. F, 65, 133-135.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.97

Cloned(Commentary)

Cloned (Comment) Organism
expression of the isolated catalytic domain in Escherichia coli strain BL21 Star (DE3) Streptococcus pneumoniae TIGR4

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant isolated catalytic domain, hanging drop vapour diffusion method at 19°C, 1 ml of 15 mg/ml protein in 20 mM Tris-HCl, pH 8.0, is mixed with 1 ml 25% PEG 1500. Removal of the His6-tag is unnecessary for crystallization. Crystals are cryoprotected in 1 ml 33% PEG 1500 supplemented with 6% MPD, X-ray diffraction structure determination and analysis at 2.0 A resolution Streptococcus pneumoniae TIGR4

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Streptococcus pneumoniae TIGR4 the enzyme is responsible for catalyzing the liberation of galactosyl beta-1,3-N-acetyl-D-galactosamine linked to serine or threonine residues of mucin-type glycoproteins ?
-
 ?

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae TIGR4
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 ATCC BAA-334D
-

Purification (Commentary)

Purification (Comment) Organism
recombinant isolated catalytic domain from Escherichia coli strain BL21 Star (DE3) Streptococcus pneumoniae TIGR4

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside + H2O
-
 Streptococcus pneumoniae TIGR4 4-nitrophenol + 3-O-beta-D-galactopyranosyl-2-acetamido-2-deoxy-alpha-D-galactopyranose
-
 ?
additional information the enzyme is responsible for catalyzing the liberation of galactosyl beta-1,3-N-acetyl-D-galactosamine linked to serine or threonine residues of mucin-type glycoproteins Streptococcus pneumoniae TIGR4 ?
-
 ?

Synonyms

Synonyms Comment Organism
endo-beta-N-acetylgalactosaminidase
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 Streptococcus pneumoniae TIGR4
More the enzyme belongs to the glycoside hydrolase family 101, GH101 Streptococcus pneumoniae TIGR4

General Information

General Information Comment Organism
physiological function the enzyme is responsible for catalyzing the liberation of galactosyl beta-1,3-N-acetyl-D-galactosamine linked to serine or threonine residues of mucin-type glycoproteins Streptococcus pneumoniae TIGR4