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Literature summary for 3.2.1.95 extracted from

  • Mizuno, T.; Mori, H.; Ito, H.; Matsui, H.; Kimura, A.; Chiba, S.
    Molecular cloning of isomaltotrio-dextranase gene from Brevibacterium fuscum var. dextranlyticum strain 0407 and its expression in Escherichia coli (1999), Biosci. Biotechnol. Biochem., 63, 1582-1588.
    View publication on PubMed
Search for more literature for 3.2.1.95

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Brevibacterium fuscum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km: 0.72 mg/ml for the recombinant enzyme and 0.26 mg/ml for the native enzyme Brevibacterium fuscum

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
 x * 68000, SDS-PAGE Brevibacterium fuscum

Organism

Organism UniProt Comment Textmining
Brevibacterium fuscum
-
 strain 0407
-
Brevibacterium fuscum
-
 var. dextranlyticum
-
Brevibacterium fuscum 407
-
 strain 0407
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Brevibacterium fuscum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
21.5
-
 recombinant enzyme Brevibacterium fuscum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dextran + H2O
-
 Brevibacterium fuscum isomaltotriose
-
 ?
dextran + H2O
-
 Brevibacterium fuscum 407 isomaltotriose
-
 ?

Subunits

Subunits Comment Organism
? x * 68000, SDS-PAGE Brevibacterium fuscum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 recombinant enzyme Brevibacterium fuscum