Protein Variants | Comment | Organism |
---|---|---|
D198N | 0.003% of wild-type activity, largely increased Kd-value | Arthrobacter globiformis |
D266N | 0.011% of wild-type activity, without change in substrate binding ability | Arthrobacter globiformis |
D313N | 0.002% of wild-type activity, largely increased Kd-value | Arthrobacter globiformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter globiformis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Arthrobacter globiformis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
isomaltotriose + H2O = alpha-isomaltose + D-glucose | D266 acts as a general acid, D198 acts as both nucleophile in the catalytic process and +binding the substrate, D313 acts in substrate binding | Arthrobacter globiformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dextran + H2O | - |
Arthrobacter globiformis | alpha-isomaltose + O-alpha-D-Glcp-(1-3)-O-alpha-D-Glcp-(1-6)-D-Glc + O-alpha-D-Glcp-(1-4)-O-alpha-D-Glcp-(1-6)-D-Glc + O-alpha-D-Glcp-(1-2)-O-alpha-D-Glcp-(1-6)-D-Glc | - |
? |