Literature summary for 3.2.1.93 extracted from

Chen, J.H.; Chi, M.C.; Lin, M.G.; Lin, L.L.; Wang, T.F.
Beneficial effect of sugar osmolytes on the refolding of guanidine hydrochloride-denatured trehalose-6-phosphate hydrolase from Bacillus licheniformis (2015), BioMed Res. Int., 2015, 806847 .

Organism

Organism	UniProt	Comment	Textmining
Bacillus licheniformis	-	-	-
Bacillus licheniformis	Q65MI2	-	-
Bacillus licheniformis 168	Q65MI2	-	-

Renatured (Commentary)

Renatured (Comment)	Organism
sorbitol, sucrose, and trehalose at a concentration of 0.75 M improve the refolding yields of guanidine hydrochloride-denatured BlTreA, probably due to the fact that these sugars favor the formation of tertiary architectures. Sorbitol is more effective than any of the other two sugars	Bacillus licheniformis
sorbitol, sucrose, and trehalose at a concentration of 0.75 M improve the refolding yields of guanidine hydrochloride-denatured enzyme. Far-UV CD measurements demonstrate the ability of sugar osmolytes to shift the secondary structure of guanidine hydrochloride-denatured enzyme towards near-native conformations. ANS fluorescence intensity measurements reveal a reduction of exposed hydrophobic surfaces upon the treatment of denatured enzyme with sugar osmolytes	Bacillus licheniformis

Renatured (Comment)

Organism

sorbitol, sucrose, and trehalose at a concentration of 0.75 M improve the refolding yields of guanidine hydrochloride-denatured BlTreA, probably due to the fact that these sugars favor the formation of tertiary architectures. Sorbitol is more effective than any of the other two sugars

Bacillus licheniformis

sorbitol, sucrose, and trehalose at a concentration of 0.75 M improve the refolding yields of guanidine hydrochloride-denatured enzyme. Far-UV CD measurements demonstrate the ability of sugar osmolytes to shift the secondary structure of guanidine hydrochloride-denatured enzyme towards near-native conformations. ANS fluorescence intensity measurements reveal a reduction of exposed hydrophobic surfaces upon the treatment of denatured enzyme with sugar osmolytes

Bacillus licheniformis

Synonyms

Synonyms	Comment	Organism
BlTreA	-	Bacillus licheniformis
trehalose-6-phosphate hydrolase	-	Bacillus licheniformis

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Release 2023.1 (January 2023)