Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.91 extracted from

  • Becker, D.; Johnson, K.S.; Koivula, A.; Schulein, M.; Sinnott, M.L.
    Hydrolyses of alpha- and beta-cellobiosyl fluorides by Cel6A (cellobiohydrolase II) of Trichoderma reesei and Humicola insolens (2000), Biochem. J., 345, 315-319.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
beta-cellobiosyl fluoride pH 5, 20°C Humicola insolens
0.15
-
beta-cellobiosyl fluoride pH 5, 20°C Trichoderma reesei
0.77
-
alpha-cellobiosyl fluoride pH 5, 20°C Humicola insolens
2.35
-
alpha-cellobiosyl fluoride pH 5, 20°C Trichoderma reesei

Organism

Organism UniProt Comment Textmining
Humicola insolens
-
-
-
Trichoderma reesei
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-cellobiosyl fluoride + H2O
-
Humicola insolens fluoride + cellobiose
-
?
alpha-cellobiosyl fluoride + H2O
-
Trichoderma reesei fluoride + cellobiose
-
?
beta-cellobiosyl fluoride + H2O
-
Humicola insolens fluoride + cellobiose
-
?
beta-cellobiosyl fluoride + H2O
-
Trichoderma reesei fluoride + cellobiose
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.01
-
alpha-cellobiosyl fluoride pH 5, 20°C Humicola insolens
0.071
-
beta-cellobiosyl fluoride pH 5, 20°C Humicola insolens
0.54
-
alpha-cellobiosyl fluoride pH 5, 20°C Trichoderma reesei
4
-
beta-cellobiosyl fluoride pH 5, 20°C Trichoderma reesei