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Literature summary for 3.2.1.91 extracted from

  • Singh, R.N.; Akimenko, V.K.
    Isolation of a cellobiohydrolase of Clostridium thermocellum capable of degrading natural crystalline substrates (1993), Biochem. Biophys. Res. Commun., 192, 1123-1130.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloned in Escherichia coli Acetivibrio thermocellus

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
Acetivibrio thermocellus
ethanol
-
Acetivibrio thermocellus
Fe3+
-
Acetivibrio thermocellus
Hg2+
-
Acetivibrio thermocellus
SDS
-
Acetivibrio thermocellus
Zn2+
-
Acetivibrio thermocellus

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus
-
CBH III
-
Acetivibrio thermocellus CBH III
-
CBH III
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4
-
cellulose Acetivibrio thermocellus
1.8
-
xylan Acetivibrio thermocellus
2.1
-
avicel Acetivibrio thermocellus
18
-
p-nitrophenyl lactose Acetivibrio thermocellus
18.7
-
p-nitrophenyl-beta-D-cellobioside Acetivibrio thermocellus
18.8
-
carboxymethyl cellulose Acetivibrio thermocellus
24.6
-
lichenan Acetivibrio thermocellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
avicel + H2O
-
Acetivibrio thermocellus cellobiose
-
?
avicel + H2O
-
Acetivibrio thermocellus CBH III cellobiose
-
?
carboxymethylcellulose + H2O
-
Acetivibrio thermocellus glucose + cellobiose + cellutetraose
-
?
carboxymethylcellulose + H2O
-
Acetivibrio thermocellus CBH III glucose + cellobiose + cellutetraose
-
?
cellulose + H2O
-
Acetivibrio thermocellus cellobiose + D-glucose + cellotriose
-
?
cellulose + H2O
-
Acetivibrio thermocellus CBH III cellobiose + D-glucose + cellotriose
-
?
lichenan + H2O
-
Acetivibrio thermocellus ?
-
?
lichenan + H2O
-
Acetivibrio thermocellus CBH III ?
-
?
p-nitrophenyl-beta-D-cellobioside + H2O
-
Acetivibrio thermocellus p-nitrophenol + cellobiose
-
?
p-nitrophenyl-lactoside + H2O
-
Acetivibrio thermocellus p-nitrophenol + lactose
-
?
p-nitrophenyl-lactoside + H2O
-
Acetivibrio thermocellus CBH III p-nitrophenol + lactose
-
?
xylan + H2O
-
Acetivibrio thermocellus xylobiose + xylotetraose + xylohexaose
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Acetivibrio thermocellus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Acetivibrio thermocellus