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Literature summary for 3.2.1.84 extracted from

  • Wiater, A.; Pleszczynska, M.; Rogalski, J.; Szajnecka, L.; Szczodrak, J.
    Purification and properties of an alpha-(1->3)-glucanase (EC 3.2.1.84) from Trichoderma harzianum and its use for reduction of artificial dental plaque accumulation (2013), Acta Biochim. Pol., 60, 123-128.
    View publication on PubMed
Search for more literature for 3.2.1.84

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ moderate inhibition at 1 mM Trichoderma harzianum
Co2+ moderate inhibition at 1 mM Trichoderma harzianum
Cu2+ moderate inhibition at 1 mM Trichoderma harzianum
Fe3+ moderate inhibition at 1 mM Trichoderma harzianum
Hg2+ complete inhibition at 1 mM Trichoderma harzianum
Mn2+ moderate inhibition at 1 mM Trichoderma harzianum
Sn2+ moderate inhibition at 1 mM Trichoderma harzianum
Zn2+ moderate inhibition at 1 mM Trichoderma harzianum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km is 0.73 mg/ml with lyophilized Laetiporus sulphureus cell walls, pH 5.5, 45°C Trichoderma harzianum

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Trichoderma harzianum
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates slightly Trichoderma harzianum
additional information no or poor effects by Ba2+, K+, and Fe2+ Trichoderma harzianum
Na+ activates slightly Trichoderma harzianum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
 x * 67000, SDS-PAGE Trichoderma harzianum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Trichoderma harzianum the enzyme preferentially catalyzes the hydrolysis of various streptococcal mutans and fungal alpha-(1->3)-glucans ?
-
 ?
additional information Trichoderma harzianum CCM F-340 the enzyme preferentially catalyzes the hydrolysis of various streptococcal mutans and fungal alpha-(1->3)-glucans ?
-
 ?

Organism

Organism UniProt Comment Textmining
Trichoderma harzianum
-
-
-
Trichoderma harzianum CCM F-340
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme 18.4fold by ultrafiltration, anion exchange and hydrophobic interaction chromatography, and chromatofocusing to homogeneity Trichoderma harzianum

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Trichoderma harzianum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
115.6
-
 purified enzyme with lyophilized Laetiporus sulphureus cell walls, pH 5.5, 45°C Trichoderma harzianum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme preferentially catalyzes the hydrolysis of various streptococcal mutans and fungal alpha-(1->3)-glucans Trichoderma harzianum ?
-
 ?
additional information alpha-(1,3)-endo-glucanase activity with lyophilized Laetiporus sulphureus cell walls prepared from fruiting bodies, also glucans are iso­lated from fruiting bodies of Pleurotus precoce, Pleurotus djamor, Pleurotus citrinopileatus, Pleurotus eryngii, Piptoporus betulinus, Laetiporus sul­phureus and Hericium erinaceus, and from mycelia of Aspergillus fumigatus CCM F-614, Aspergillus wentii CIM 449, and Aspergillus nidulans DIM AN1, and with dextranase-pretreated mutan synthesized from su­crose using cell-free glucosyltransferases of Streptococci, substrate specificity, overview Trichoderma harzianum ?
-
 ?
additional information the enzyme preferentially catalyzes the hydrolysis of various streptococcal mutans and fungal alpha-(1->3)-glucans Trichoderma harzianum CCM F-340 ?
-
 ?
additional information alpha-(1,3)-endo-glucanase activity with lyophilized Laetiporus sulphureus cell walls prepared from fruiting bodies, also glucans are iso­lated from fruiting bodies of Pleurotus precoce, Pleurotus djamor, Pleurotus citrinopileatus, Pleurotus eryngii, Piptoporus betulinus, Laetiporus sul­phureus and Hericium erinaceus, and from mycelia of Aspergillus fumigatus CCM F-614, Aspergillus wentii CIM 449, and Aspergillus nidulans DIM AN1, and with dextranase-pretreated mutan synthesized from su­crose using cell-free glucosyltransferases of Streptococci, substrate specificity, overview Trichoderma harzianum CCM F-340 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 67000, SDS-PAGE Trichoderma harzianum

Synonyms

Synonyms Comment Organism
alpha-(1->3)-glucanase
-
 Trichoderma harzianum
mutanase
-
 Trichoderma harzianum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Trichoderma harzianum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 purified enzyme, pH 5.5, 1 h, stable Trichoderma harzianum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Trichoderma harzianum

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 6 purified enzyme, 45°C, 1 h, stable at Trichoderma harzianum

pI Value

Organism Comment pI Value Maximum pI Value
Trichoderma harzianum isoelectric focusing
-
 7.1