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Literature summary for 3.2.1.84 extracted from

  • Ait-Lahsen, H.; Soler, A.; Rey, M.; de La Cruz, J.; Monte, E.; Llobell, A.
    An antifungal exo-alpha-1,3-glucanase (AGN13.1) from the biocontrol fungus Trichoderma harzianum (2001), Appl. Environ. Microbiol., 67, 5833-5839.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.84

Application

Application Comment Organism
biotechnology transgenic plants might have improved resistance to fungal pathogens Trichoderma harzianum

Cloned(Commentary)

Cloned (Comment) Organism
-
 Trichoderma harzianum

General Stability

General Stability Organism
alpha-1,3-glucan stabilizes the enzyme Trichoderma harzianum

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Trichoderma harzianum
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63800
-
 calculated from amino acid sequence Trichoderma harzianum
67000
-
 gel filtration Trichoderma harzianum
72000
-
 SDS-PAGE Trichoderma harzianum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Trichoderma harzianum lytic and antifungal activity against fungal plant panthogens ?
-
 ?
additional information Trichoderma harzianum CECT2413 lytic and antifungal activity against fungal plant panthogens ?
-
 ?

Organism

Organism UniProt Comment Textmining
Trichoderma harzianum
-
 sequence was found to be identical to previously published data
-
Trichoderma harzianum CECT2413
-
 sequence was found to be identical to previously published data
-

Purification (Commentary)

Purification (Comment) Organism
-
 Trichoderma harzianum

Storage Stability

Storage Stability Organism
-20°C, 100 mM acetate buffer, pH 5.5 Trichoderma harzianum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-1,3-glucan + H2O
-
 Trichoderma harzianum alpha-D-glucose
-
 ?
alpha-1,3-glucan + H2O
-
 Trichoderma harzianum CECT2413 alpha-D-glucose
-
 ?
alpha-1,3-mutan + H2O
-
 Trichoderma harzianum alpha-D-glucose
-
 ?
alpha-1,3-mutan + H2O
-
 Trichoderma harzianum CECT2413 alpha-D-glucose
-
 ?
beta-glucan:chitin + H2O different activities with substrates from cell walls of different microorganisms Trichoderma harzianum ?
-
 ?
beta-glucan:chitin + H2O different activities with substrates from cell walls of different microorganisms Trichoderma harzianum CECT2413 ?
-
 ?
additional information lytic and antifungal activity against fungal plant panthogens Trichoderma harzianum ?
-
 ?
additional information no activity with beta-glucan or alpha-1,4 or alpha-1,6 linked glucose chains Trichoderma harzianum ?
-
 ?
additional information lytic and antifungal activity against fungal plant panthogens Trichoderma harzianum CECT2413 ?
-
 ?
additional information no activity with beta-glucan or alpha-1,4 or alpha-1,6 linked glucose chains Trichoderma harzianum CECT2413 ?
-
 ?

Subunits

Subunits Comment Organism
monomer equal molecular weight determined with SDS-PAGE (72000 Da) and gel filtration (67000 Da) Trichoderma harzianum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Trichoderma harzianum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Trichoderma harzianum

pI Value

Organism Comment pI Value Maximum pI Value
Trichoderma harzianum isoelectric focusing
-
 7.1
Trichoderma harzianum basic chromatofocusing
-
 7.5