Any feedback?
Literature summary for 3.2.1.82 extracted from
- Molecular cloning and sequencing of the gene encoding an exopolygalacturonase of a Bacillus isolate and properties of its recombinant enzyme (2001), Biochim. Biophys. Acta, 1568, 162-170.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Bacillus sp. (in: Bacteria) |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 0.4 mM Ca2+ shifts the optimal temperature from 50°C to 55°C, the activity is 1.2fold higher than the maximal activity without Ca2+ at 50°C | Bacillus sp. (in: Bacteria) |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 105000 | - |
x * 105000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus sp. (in: Bacteria) | Q8RR74 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus sp. (in: Bacteria) |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture medium | - |
Bacillus sp. (in: Bacteria) | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 33.1 | - |
- |
Bacillus sp. (in: Bacteria) |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme is unable to degrade citrus pectins with degrees of esterification of 63% and 93% | Bacillus sp. (in: Bacteria) | ? | - |
? | |
| oligogalacturonate + H2O | - |
Bacillus sp. (in: Bacteria) | digalacturonate | - |
? | |
| polygalacturonate + H2O | - |
Bacillus sp. (in: Bacteria) | digalacturonate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 105000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| exo-PHase | - |
Bacillus sp. (in: Bacteria) |
| exopolygalacturonase | - |
Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
Tris-HCl buffer in presence of Ca2+ | Bacillus sp. (in: Bacteria) |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 65 | 30°C: about 30% of maximal activity, 65°C: about 30% of maximal activity | Bacillus sp. (in: Bacteria) |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
pH 7.0, 15 min, stable up to | Bacillus sp. (in: Bacteria) |
| 60 | - |
pH 7.0, 15 min, about 5% loss of activity | Bacillus sp. (in: Bacteria) |
| 65 | - |
pH 7.0, 15 min, about 90% loss of activity | Bacillus sp. (in: Bacteria) |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 10 | pH 7.0: about 55% of maximal activity, pH 10.0: about 35% of maximal activity | Bacillus sp. (in: Bacteria) |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Bacillus sp. (in: Bacteria) | isoelectrofocusing, pH-gradient 3.5-9.5 | - |
5 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
