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Literature summary for 3.2.1.81 extracted from
- Production and purification of agarase from a marine agarolytic bacterium Agarivorans sp. HZ105 (2009), J. Appl. Microbiol., 106, 181-190.
Application
| Application | Comment | Organism |
|---|---|---|
| industry | strain HZ105 may be useful to produce stable agarases | Agarivorans sp. HZ105 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | no agarase activity at a 6% NaCl concentration | Agarivorans sp. HZ105 |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | is required for activity, good production of agarase at NaCl concentration range from 1.5-3%. Maximum agarase production at 2.5% NaCl concentration | Agarivorans sp. HZ105 | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
presence of three extracellular agarases (HZ-a, HZ-b and HZ-c), 1 * 58000, SDS-PAGE of HZ-b, 1 * 54000, SDS-PAGE of HZ-b | Agarivorans sp. HZ105 |
| 58000 | - |
presence of three extracellular agarases (HZ-a, HZ-b and HZ-c), 1 * 58000, SDS-PAGE of HZ-b, 1 * 54000, SDS-PAGE of HZ-b | Agarivorans sp. HZ105 |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Agarivorans sp. HZ105 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity directly from the native-PAGE without stained by Coomassie brilliant blue, agarase Hz-b 18.6fold purified and agarase Hz-c 13.9fold purified | Agarivorans sp. HZ105 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.13 | - |
concentrated agarase solution | Agarivorans sp. HZ105 |
| 57.45 | - |
agarase Hz-c, 13.9fold purified | Agarivorans sp. HZ105 |
| 76.8 | - |
agarase Hz-b, 18.6fold purified | Agarivorans sp. HZ105 |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 25°C, 2 days | Agarivorans sp. HZ105 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agar + H2O | - |
Agarivorans sp. HZ105 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | presence of three extracellular agarases (HZ-a, HZ-b and HZ-c), 1 * 58000, SDS-PAGE of HZ-b, 1 * 54000, SDS-PAGE of HZ-b | Agarivorans sp. HZ105 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AgaA | - |
Agarivorans sp. HZ105 |
| agarase | - |
Agarivorans sp. HZ105 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Agarivorans sp. HZ105 |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | - |
Agarivorans sp. HZ105 |
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