Cloned (Comment) | Organism |
---|---|
gene FEH-III, DNA and amino acid sequence determination and analysis, FEH isozyme sequences comparisons, phylogenetic analysis, functional recombinant expression of His-tagged enzyme in Pichia pastoris strain X-33 | Helianthus tuberosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helianthus tuberosus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the Ht1-FEH III sequence harbors four potential N-glycosylation sites | Helianthus tuberosus |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Pichia pastoris strain X-33 by nickel affinity chromatography and ultrafiltration | Helianthus tuberosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-kestose + H2O | best substrate | Helianthus tuberosus | beta-D-fructose + sucrose | - |
? | |
fructofuranosylnystose + H2O | high activity | Helianthus tuberosus | ? | - |
? | |
inulin + H2O | high activity | Helianthus tuberosus | ? | - |
? | |
levan + H2O | low to moderate activity | Helianthus tuberosus | ? | - |
? | |
additional information | substrate specificity, overview | Helianthus tuberosus | ? | - |
? | |
nystose + H2O | - |
Helianthus tuberosus | ? | - |
? | |
sucrose + H2O | - |
Helianthus tuberosus | D-glucose + D-fructose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 64700, recombinant His-tagged enzyme, SDS-PAGE | Helianthus tuberosus |
Synonyms | Comment | Organism |
---|---|---|
Ht1-FEH III | - |
Helianthus tuberosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Helianthus tuberosus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 60 | over 50% of maximal activity within this range | Helianthus tuberosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Helianthus tuberosus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 6.5 | over 60% of maximal activity within this range | Helianthus tuberosus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Helianthus tuberosus | sequence calculation | - |
6.45 |
General Information | Comment | Organism |
---|---|---|
evolution | phylogenetic analysis of deduced amino acid sequences of plant fructan metabolism genes | Helianthus tuberosus |
additional information | the Ht1-FEH III sequence harbors three conserved amino acid motifs (S/NDPNG, FRDP, and ECPD) and four potential N-glycosylation sites | Helianthus tuberosus |
physiological function | the third member of the Ht1-FEH family in Jerusalem artichoke, i.e., Ht1-FEH III, not only demonstrates hydrolysis activity towards beta-(2->1)-linked fructans and beta-(2->6)-linked levan, but also towards sucrose | Helianthus tuberosus |