Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris | Phleum pratense |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
sucrose | the inhibitory effect of sucrose is 4% at 10 mM sucrose and 23% at 100 mM sucrose | Phleum pratense |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63400 | - |
x * 63400, calculated from amino acid sequence | Phleum pratense |
78000 | - |
x * 78000, SDS-PAGE | Phleum pratense |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phleum pratense | - |
cultivar Hokushu | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
plant crown | - |
Phleum pratense | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,1,1-kestopentaose + H2O | 18% activity compared to levan | Phleum pratense | ? | - |
? | |
1,1-kestotetraose + H2O | 18% activity compared to levan | Phleum pratense | ? | - |
? | |
1-kestotriose + H2O | 7% activity compared to levan | Phleum pratense | ? | - |
? | |
6-kestotriose + H2O | 25% activity compared to levan | Phleum pratense | ? | - |
? | |
inulin + 5 H2O | 6% activity compared to levan | Phleum pratense | 5 beta-D-fructofuranose + alpha-D-glucopyranose | - |
? | |
levan + H2O | 100% activity | Phleum pratense | beta-D-fructose + ? | - |
? | |
additional information | the recombinant enzyme completely degrades fructans that are composed mainly of beta(2,6)-linked and linear fructans (levan) with a high degree of polymerization. The enzyme has significant activity against beta(2,1)-linked fructans, but considerably less than against beta(2,6)-linked fructans. The enzyme also has weak invertase activity | Phleum pratense | ? | - |
? | |
sucrose + H2O | less than 1%, 2% and 15% activity towards 1 mM, 10 mM and 100 mM sucrose compared to levan | Phleum pratense | D-fructose + D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 78000, SDS-PAGE | Phleum pratense |
? | x * 63400, calculated from amino acid sequence | Phleum pratense |
Synonyms | Comment | Organism |
---|---|---|
FEH1 | - |
Phleum pratense |
fructan 1-exohydrolase | - |
Phleum pratense |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Phleum pratense |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
the activity of the enzyme remains relatively high even at 4°C, with up to 38% of its activity at 30°C | Phleum pratense |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Phleum pratense |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Phleum pratense | calculated from amino acid sequence | - |
5 |
Organism | Comment | Expression |
---|---|---|
Phleum pratense | in the stubble of seedlings and in young haplocorms from adult timothy plants, enzyme transcripts are significantly increased within 3 h of defoliation | up |