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Literature summary for 3.2.1.80 extracted from

  • Tamura, K.I.; Sanada, Y.; Tase, K.; Komatsu, T.; Yoshida, M.
    Pp6-FEH1 encodes an enzyme for degradation of highly polymerized levan and is transcriptionally induced by defoliation in timothy (Phleum pratense L.) (2011), J. Exp. Bot., 62, 3421-3431.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Phleum pratense

Inhibitors

Inhibitors Comment Organism Structure
sucrose the inhibitory effect of sucrose is 4% at 10 mM sucrose and 23% at 100 mM sucrose Phleum pratense

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63400
-
x * 63400, calculated from amino acid sequence Phleum pratense
78000
-
x * 78000, SDS-PAGE Phleum pratense

Organism

Organism UniProt Comment Textmining
Phleum pratense
-
cultivar Hokushu
-

Source Tissue

Source Tissue Comment Organism Textmining
plant crown
-
Phleum pratense
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,1,1-kestopentaose + H2O 18% activity compared to levan Phleum pratense ?
-
?
1,1-kestotetraose + H2O 18% activity compared to levan Phleum pratense ?
-
?
1-kestotriose + H2O 7% activity compared to levan Phleum pratense ?
-
?
6-kestotriose + H2O 25% activity compared to levan Phleum pratense ?
-
?
inulin + 5 H2O 6% activity compared to levan Phleum pratense 5 beta-D-fructofuranose + alpha-D-glucopyranose
-
?
levan + H2O 100% activity Phleum pratense beta-D-fructose + ?
-
?
additional information the recombinant enzyme completely degrades fructans that are composed mainly of beta(2,6)-linked and linear fructans (levan) with a high degree of polymerization. The enzyme has significant activity against beta(2,1)-linked fructans, but considerably less than against beta(2,6)-linked fructans. The enzyme also has weak invertase activity Phleum pratense ?
-
?
sucrose + H2O less than 1%, 2% and 15% activity towards 1 mM, 10 mM and 100 mM sucrose compared to levan Phleum pratense D-fructose + D-glucose
-
?

Subunits

Subunits Comment Organism
? x * 78000, SDS-PAGE Phleum pratense
? x * 63400, calculated from amino acid sequence Phleum pratense

Synonyms

Synonyms Comment Organism
FEH1
-
Phleum pratense
fructan 1-exohydrolase
-
Phleum pratense

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Phleum pratense

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
the activity of the enzyme remains relatively high even at 4°C, with up to 38% of its activity at 30°C Phleum pratense

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Phleum pratense

pI Value

Organism Comment pI Value Maximum pI Value
Phleum pratense calculated from amino acid sequence
-
5

Expression

Organism Comment Expression
Phleum pratense in the stubble of seedlings and in young haplocorms from adult timothy plants, enzyme transcripts are significantly increased within 3 h of defoliation up