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Literature summary for 3.2.1.8 extracted from

  • Xu, B.; Dai, L.; Li, J.; Deng, M.; Miao, H.; Zhou, J.; Mu, Y.; Wu, Q.; Tang, X.; Yang, Y.; Ding, J.; Han, N.; Huang, Z.
    Molecular and biochemical characterization of a novel xylanase from Massilia sp. RBM26 isolated from the feces of Rhinopithecus bieti (2016), J. Microbiol. Biotechnol., 26, 9-19.
    View publication on PubMed
Search for more literature for 3.2.1.8

Activating Compound

Activating Compound Comment Organism Structure
Mn2+ 1 mM, 115% of initial activity Massilia sp. RBM26

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Massilia sp. RBM26

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM, no residual activity Massilia sp. RBM26
Hg2+ 1 mM, no residual activity Massilia sp. RBM26
NaCl 5 M, 86% residual activity Massilia sp. RBM26
SDS 1 mM, no residual activity Massilia sp. RBM26

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 beechwood xylan Km value 9.49 mg/ml, pH 5.5, 45°C Massilia sp. RBM26

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
-
 Massilia sp. RBM26

Organism

Organism UniProt Comment Textmining
Massilia sp. RBM26 A0A0S2I9J6 isolated from the feces of Rhinopithecus bieti
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beechwood xylan + H2O
-
 Massilia sp. RBM26 xylose + xylobiose + xylopentaose + ?
-
 ?
additional information no substrates: barley beta-glucan, carboxymethyl cellulose sodium salt, laminarin, microcrystalline cellulose, or 2 mmol/l 4-nitrophenyl-beta-D-xylopyranoside Massilia sp. RBM26 ?
-
 ?
oat spelt xylan + H2O
-
 Massilia sp. RBM26 xylose + xylobiose + xylopentaose + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE, x * 43170, calculated Massilia sp. RBM26

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Massilia sp. RBM26

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50 more than 60% of maximum activity Massilia sp. RBM26

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 1 h, more than 96% residual activity Massilia sp. RBM26

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
47.3
-
 beechwood xylan pH 5.5, 45°C Massilia sp. RBM26

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Massilia sp. RBM26

pH Range

pH Minimum pH Maximum Comment Organism
5 8 more than 90% of maximum activity Massilia sp. RBM26