Application | Comment | Organism |
---|---|---|
degradation | immobilization of enzyme within calcium alginate beads using entrapment technique. Temperature (50°C) and pH (7.0) optima of immobilized enzyme remain same, but enzyme-substrate reaction time increases from 5.0 to 30.0 min as compared to free enzyme. The diffusion limit of high molecular weight xylan (corncob) causes a decline in Vmax of immobilized enzyme from 4773 to 203.7 U/min, whereas the Km value increases from 0.5074 to 0.5722 mg/ml. Immobilized endo-beta-1,4-xylanase is stable even at high temperatures and retains 18 and 9% residual activity at 70°C and 80°C, respectively. The immobilized enzyme also exhibits sufficient recycling efficiency up to five reaction cycles | Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
corncob xylan | Km value of free enzyme is 0.507 mg/ml, of immobilized enzyme 0.572 mg/ml, pH 7.0, 50°C | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Geobacillus stearothermophilus KIBGE-IB29 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
corncob xylan + H2O | - |
Geobacillus stearothermophilus | xylo-oligosaccharides + ? | - |
? | |
corncob xylan + H2O | - |
Geobacillus stearothermophilus KIBGE-IB29 | xylo-oligosaccharides + ? | - |
? |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
18% of maximum activity | Geobacillus stearothermophilus |
80 | - |
9% of maximum activity | Geobacillus stearothermophilus |