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Literature summary for 3.2.1.8 extracted from
- Expression of the Thermobifida fusca xylanase Xyn11A in Pichia pastoris and its characterization (2015), BMC Biotechnol., 15, 18.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Pichia pastoris | Thermobifida fusca |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Thermobifida fusca | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermobifida fusca | Q47QL8 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 516 | - |
recombinant protein with His-tag, pH 8.0, 80°C | Thermobifida fusca |
| 557 | - |
recombinant wild-type protein, pH 8.0, 80°C | Thermobifida fusca |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beechwood xylan + H2O | - |
Thermobifida fusca | xylotriose + xylotetraose + xylopentaose + ? | about 19.9% xylobiose, 33.6% xylotetraose, 40.6% xylopentaose plus some xylose and xylobiose | ? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Tfu_1213 | - |
Thermobifida fusca |
| Xyn11A | - |
Thermobifida fusca |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
recombinant protein, both with and without His-tag | Thermobifida fusca |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 80 | more than 60% of maximum activity, recombinant protein, both with and without His-tag | Thermobifida fusca |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
30 min, more than 10% residual activity, recombinant protein, both with and without His-tag | Thermobifida fusca |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
recombinant protein, both with and without His-tag | Thermobifida fusca |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | more than 60% of maximum activity, recombinant protein, both with and without His-tag | Thermobifida fusca |
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