Application | Comment | Organism |
---|---|---|
degradation | immobilizazion of enzyme on various supports. Most active immobilized enzyme is achieved when Xyl2 is covalently bound to low functionalized agarose matrices, poorer activity is observed for Xyl2 immobilized on highly functionalized agarose or on nickel-affinity resin | Fusarium oxysporum f. sp. vasinfectum |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Fusarium oxysporum f. sp. vasinfectum |
Crystallization (Comment) | Organism |
---|---|
Xyl2 at 1.56 A resolution and in complex with methyl-xylopyranoside at 2.84 A resolution. Xyl2 folds into the characteristic GH11 xylanase fold consisting of a highly twisted beta-sandwich core that comprises 14 beta-strands and is further stabilized by a single alpha-helix in the opposite side of the active site. The hydrogen bonding interactions between methyl-xylopyranoside and residues Glu85, Ser126, Tyr170 and Tyr176 are all located in a cavity encircled by the side chains of Pro5 and the side chains of Trp18, Asn44 and Pro125 | Fusarium oxysporum f. sp. vasinfectum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
40 | - |
4-O-methyl-D-glucurono-D-xylan | pH 8.0, 40°C | Fusarium oxysporum f. sp. vasinfectum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activation by divalent cation up to 40% at 1 mM, in decreasing order of activation effect: Mg2+, Ni2+, Co2+, Ca2+, Mn2+ and Zn2+ | Fusarium oxysporum f. sp. vasinfectum | |
Co2+ | activation by divalent cation up to 40% at 1 mM, in decreasing order of activation effect: Mg2+, Ni2+, Co2+, Ca2+, Mn2+ and Zn2+ | Fusarium oxysporum f. sp. vasinfectum | |
Mg2+ | activation by divalent cation up to 40% at 1 mM, in decreasing order of activation effect: Mg2+, Ni2+, Co2+, Ca2+, Mn2+ and Zn2+ | Fusarium oxysporum f. sp. vasinfectum | |
Mn2+ | activation by divalent cation up to 40% at 1 mM, in decreasing order of activation effect: Mg2+, Ni2+, Co2+, Ca2+, Mn2+ and Zn2+ | Fusarium oxysporum f. sp. vasinfectum | |
Ni2+ | activation by divalent cation up to 40% at 1 mM, in decreasing order of activation effect: Mg2+, Ni2+, Co2+, Ca2+, Mn2+ and Zn2+ | Fusarium oxysporum f. sp. vasinfectum | |
Zn2+ | activation by divalent cation up to 40% at 1 mM, in decreasing order of activation effect: Mg2+, Ni2+, Co2+, Ca2+, Mn2+ and Zn2+ | Fusarium oxysporum f. sp. vasinfectum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21200 | - |
- |
Fusarium oxysporum f. sp. vasinfectum |
Organic Solvent | Comment | Organism |
---|---|---|
1-ethyl-3-methylimidazolium acetate | ionic liquid, no inhibition up to 15% w/v | Fusarium oxysporum f. sp. vasinfectum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fusarium oxysporum f. sp. vasinfectum | X0M5X0 | - |
- |
Fusarium oxysporum f. sp. vasinfectum 25433 | X0M5X0 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-O-methyl-D-glucurono-D-xylan + H2O | substrate dyed with Remazol brilliant blue R | Fusarium oxysporum f. sp. vasinfectum | xylo-oligosaccharides + ? | - |
? | |
4-O-methyl-D-glucurono-D-xylan + H2O | substrate dyed with Remazol brilliant blue R | Fusarium oxysporum f. sp. vasinfectum 25433 | xylo-oligosaccharides + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 21200, SDS-PAGE | Fusarium oxysporum f. sp. vasinfectum |
Synonyms | Comment | Organism |
---|---|---|
FOTG_15646 | - |
Fusarium oxysporum f. sp. vasinfectum |
XYL2 | - |
Fusarium oxysporum f. sp. vasinfectum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
4-O-methyl-D-glucurono-D-xylan | pH 8.0, 40°C | Fusarium oxysporum f. sp. vasinfectum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | substrate 4-O-methyl-D-glucurono-D-xylan | Fusarium oxysporum f. sp. vasinfectum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.27 | - |
4-O-methyl-D-glucurono-D-xylan | pH 8.0, 40°C | Fusarium oxysporum f. sp. vasinfectum |