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Literature summary for 3.2.1.8 extracted from
- Construction of thermophilic xylanase and its structural analysis (2016), Biochemistry, 55, 4399-4409.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| mutant S35C/N44H/Y61M/T62C/N63L/D65P/N66G/T101P/S102N, to 2.0 A resolution. The formation of disulfide bond S35C/T62C induces new C-C contacts and a conformational change in the N-terminus. The induced conformational change is key for stabilizing the N-terminal region and for constructing thermostable mutants without compromising the activity | Talaromyces pinophilus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D65P | 98% of wild-type activity, decrease in melting temperature | Talaromyces pinophilus |
| D65P/N66G | 102% of wild-type activity, increase in melting temperature | Talaromyces pinophilus |
| N44H | 101%% of wild-type activity, decrease in melting temperature | Talaromyces pinophilus |
| N63L | 85% of wild-type activity, decrease in melting temperature | Talaromyces pinophilus |
| N66G | 100% of wild-type activity, decrease in melting temperature | Talaromyces pinophilus |
| S102N | 100%% of wild-type activity, increase in melting temperature | Talaromyces pinophilus |
| S35C | 101% % of wild-type activity, decrease in melting temperature | Talaromyces pinophilus |
| S35C/N44H/Y61M/T62C/N63L/D65P/N66G/T101P/S102N | 114% of wild-type activity, 20 degrees increase in melting temperature | Talaromyces pinophilus |
| S35C/T62C | 97%% of wild-type activity, increase in melting temperature | Talaromyces pinophilus |
| T101P | 99%% of wild-type activity | Talaromyces pinophilus |
| T62C | 86%% of wild-type activity, decrease in melting temperature | Talaromyces pinophilus |
| Y61M | 94% of wild-type activity, decrease in melting temperature | Talaromyces pinophilus |
| Y61M/N63L | 109% of wild-type activity, decrease in melting temperature | Talaromyces pinophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Talaromyces pinophilus | W8VR85 | - |
- |
| Talaromyces pinophilus CF-2612 | W8VR85 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3991 | - |
wild-type, pH 5.0, 50°C | Talaromyces pinophilus |
| 4659 | - |
mutant S35C/N44H/Y61M/T62C/N63L/D65P/N66G/T101P/S102N, pH 5.0, 50°C | Talaromyces pinophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| birchwood xylan + H2O | - |
Talaromyces pinophilus | xylo-oligosaccharides + ? | - |
? |  |
| birchwood xylan + H2O | - |
Talaromyces pinophilus CF-2612 | xylo-oligosaccharides + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| XylC | - |
Talaromyces pinophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 58.9 | - |
wild-type, melting temperature | Talaromyces pinophilus |
| 64.6 | - |
mutant D65P/N66G, melting temperature | Talaromyces pinophilus |
| 74.5 | - |
mutant S35C/T62C, melting temperature | Talaromyces pinophilus |
| 80.5 | - |
mutant S35C/N44H/Y61M/T62C/N63L/D65P/N66G/T101P/S102N, melting temperature | Talaromyces pinophilus |
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Release 2023.1 (January 2023)
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