Literature summary for 3.2.1.8 extracted from

Matteotti, C.; Bauwens, J.; Brasseur, C.; Tarayre, C.; Thonart, P.; Destain, J.; Francis, F.; Haubruge, E.; De Pauw, E.; Portetelle, D.; Vandenbol, M.
Identification and characterization of a new xylanase from Gram-positive bacteria isolated from termite gut (Reticulitermes santonensis) (2012), Protein Expr. Purif., 83, 117-127.

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Activating Compound

Activating Compound	Comment	Organism	Structure
2-mercaptoethanol	0.5%, 155% of initial activity	bacterium enrichment culture clone Xyl8B8
dithiothreitol	0.5%, 240% of initial activity	bacterium enrichment culture clone Xyl8B8
Tween 80	0.5%, 140% of initial activity	bacterium enrichment culture clone Xyl8B8

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	bacterium enrichment culture clone Xyl8B8

Inhibitors

Inhibitors	Comment	Organism	Structure
Cu2+	5 mM, about 60% residual activity	bacterium enrichment culture clone Xyl8B8
EDTA	1 mM, about 60% residual activity	bacterium enrichment culture clone Xyl8B8
sodium dodecylsulfate	0.5%, about 40% residual activity	bacterium enrichment culture clone Xyl8B8

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	beechwood xylan	Km value is 9 mg/ml, pH 5, 55°C	bacterium enrichment culture clone Xyl8B8

Organism

Organism	UniProt	Comment	Textmining
bacterium enrichment culture clone Xyl8B8	H9ZGD1	bacterium enrichment culture clone Xyl8B8	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein	bacterium enrichment culture clone Xyl8B8

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
beechwood xylan + H2O	-	bacterium enrichment culture clone Xyl8B8	?	-	?

Synonyms

Synonyms	Comment	Organism
XylB8	-	bacterium enrichment culture clone Xyl8B8

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
55	-	-	bacterium enrichment culture clone Xyl8B8

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
70	-	65% residual activity	bacterium enrichment culture clone Xyl8B8

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
100	-	30 min, 28% residual activity	bacterium enrichment culture clone Xyl8B8

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.5	-	acetate buffer	bacterium enrichment culture clone Xyl8B8

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Release 2023.1 (January 2023)