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Literature summary for 3.2.1.8 extracted from

  • Lee, S.H.; Lee, Y.E.
    Cloning and characterization of a multidomain GH10 xylanase from Paenibacillus sp. DG-22 (2014), J. Microbiol. Biotechnol., 24, 1525-1535.
    View publication on PubMed
Search for more literature for 3.2.1.8

Cloned(Commentary)

Cloned (Comment) Organism
-
 Paenibacillus sp.

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM, 4.8% residual activity Paenibacillus sp.
EDTA 1 mM, 73% residual activity Paenibacillus sp.
Hg2+ 1 mM, 4.2% residual activity Paenibacillus sp.
Ni2+ 1 mM, 51% residual activity Paenibacillus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 wheat arabinoxylan Km value is 12.2 mg/ml, pH 6.5, 65°C Paenibacillus sp.
additional information
-
 birchwood xylan Km value is 28.3 mg/ml, pH 6.5, 65°C Paenibacillus sp.
additional information
-
 beechwood xylan Km value is 3.5 mg/ml, pH 6.5, 65°C Paenibacillus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
156700
-
 x * 156700, calculated Paenibacillus sp.

Organism

Organism UniProt Comment Textmining
Paenibacillus sp. A0A075EEX4
-
-
Paenibacillus sp. DG-22 A0A075EEX4
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
454
-
 substrate birchwood xylan, pH 6.5, 65°C Paenibacillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beechwood xylan + H2O 129% of the activity with birchwood xylan Paenibacillus sp. ?
-
 ?
beechwood xylan + H2O 129% of the activity with birchwood xylan Paenibacillus sp. DG-22 ?
-
 ?
birchwood xylan + H2O
-
 Paenibacillus sp. ? enzyme releases xylooligosaccharides of various length at the initial stage of xylan hydrolysis. After 2 h of incubation, the major products of hydrolysis are xylobiose, xylotriose, xylotetraose, and short-chain xylooligosaccharides ?
birchwood xylan + H2O
-
 Paenibacillus sp. DG-22 ? enzyme releases xylooligosaccharides of various length at the initial stage of xylan hydrolysis. After 2 h of incubation, the major products of hydrolysis are xylobiose, xylotriose, xylotetraose, and short-chain xylooligosaccharides ?
additional information no substrates: avicel, carboxymethyl cellulose, cellulose Paenibacillus sp. ?
-
 ?
additional information no substrates: avicel, carboxymethyl cellulose, cellulose Paenibacillus sp. DG-22 ?
-
 ?
oat spelt xylan + H2O 53% of the activity with birchwood xylan Paenibacillus sp. ?
-
 ?
oat spelt xylan + H2O 53% of the activity with birchwood xylan Paenibacillus sp. DG-22 ?
-
 ?
wheat arabinoxylan + H2O 115% of the activity with birchwood xylan Paenibacillus sp. ?
-
 ?
wheat arabinoxylan + H2O 115% of the activity with birchwood xylan Paenibacillus sp. DG-22 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 156700, calculated Paenibacillus sp.

Synonyms

Synonyms Comment Organism
beta-xylanase
-
 Paenibacillus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Paenibacillus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 4 h, 65% residual activity Paenibacillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Paenibacillus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 10
-
 Paenibacillus sp.
10
-
 45°C, 2 h, 85% residual activity Paenibacillus sp.