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Literature summary for 3.2.1.8 extracted from

  • Liao, H.; Sun, S.; Wang, P.; Bi, W.; Tan, S.; Wei, Z.; Mei, X.; Liu, D.; Raza, W.; Shen, Q.; Xu, Y.
    A new acidophilic endo-beta-1,4-xylanase from Penicillium oxalicum: cloning, purification, and insights into the influence of metal ions on xylanase activity (2014), J. Ind. Microbiol. Biotechnol., 41, 1071-1083.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Triton X-100 1%, 88% of initial activity Penicillium oxalicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
beechwood xylan Km value is 30.7 mg/ml, pGH 4.0, 50°C Penicillium oxalicum
additional information
-
oat spelt xylan Km value is 41.3 mg/ml, pGH 4.0, 50°C Penicillium oxalicum
additional information
-
birch wood xylan Km value is 51.4 mg/ml, pGH 4.0, 50°C Penicillium oxalicum

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 1 mM, 139% of initial activity Penicillium oxalicum
Ca2+ 1 mM, 131% of initial activity Penicillium oxalicum
Fe2+ 1 mM, 155% of initial activity Penicillium oxalicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21300
-
x * 21300, SDS-PAGE Penicillium oxalicum

Organism

Organism UniProt Comment Textmining
Penicillium oxalicum E7EF85
-
-
Penicillium oxalicum GZ-2 E7EF85
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Penicillium oxalicum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
60
-
substrate birch wood xylan, pH 4.0, 50°C Penicillium oxalicum
72.6
-
substrate oat spelt xylan, pH 4.0, 50°C Penicillium oxalicum
150
-
substrate beechwood xylan, pH 4.0, 50°C Penicillium oxalicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beechwood xylan + H2O
-
Penicillium oxalicum ?
-
?
beechwood xylan + H2O
-
Penicillium oxalicum GZ-2 ?
-
?
birch wood xylan + H2O
-
Penicillium oxalicum ?
-
?
birch wood xylan + H2O
-
Penicillium oxalicum GZ-2 ?
-
?
oat spelt xylan + H2O
-
Penicillium oxalicum ?
-
?
oat spelt xylan + H2O
-
Penicillium oxalicum GZ-2 ?
-
?

Subunits

Subunits Comment Organism
? x * 21300, SDS-PAGE Penicillium oxalicum

Synonyms

Synonyms Comment Organism
Xyn11A
-
Penicillium oxalicum
XynB
-
Penicillium oxalicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
Penicillium oxalicum