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Literature summary for 3.2.1.8 extracted from

  • Gao, H.; Yan, P.; Zhang, B.; Shan, A.
    Expression of Aspergillus niger IA-001 endo-beta-1,4-xylanase in Pichia pastoris and analysis of the enzymic characterization (2014), Appl. Biochem. Biotechnol., 173, 2028-2041.
    View publication on PubMed

Application

Application Comment Organism
synthesis production of enzyme in Pichia pastoris with codon optimization. The activity of dual-copy enzyme is maximized at 15158 U/ml after 120 h of shaking Aspergillus niger

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Aspergillus niger

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 10 mM, 77% residual activity Aspergillus niger
Fe2+ 10 mM, 83% residual activity Aspergillus niger
sodium dodecylsulfate 10 mM, 72% residual activity Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
xylan Km value is 18.27 mg/ml, pH 5.0, 50°C Aspergillus niger

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 10 mM, 119% of initial activity Aspergillus niger
Tween 80 0.5%, 185% of initial activity Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
x * 23000, SDS-PAGE Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger F5CI28
-
-
Aspergillus niger IA-0010 F5CI28
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6853
-
substrate birchwood xylan, pH 5.0, 50°C Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information almost no activity on xylobiose Aspergillus niger ?
-
?
additional information almost no activity on xylobiose Aspergillus niger IA-0010 ?
-
?
xylan + H2O substrate birchwood xylan Aspergillus niger xylobiose + D-xylose + ? after 24 h of hydrolysis, more than 90% of the total hydrolysis products of xylan are xylobiose and D-xylose with almost no xylotetraose, -pentaose, or -hexaose ?
xylan + H2O substrate birchwood xylan Aspergillus niger IA-0010 xylobiose + D-xylose + ? after 24 h of hydrolysis, more than 90% of the total hydrolysis products of xylan are xylobiose and D-xylose with almost no xylotetraose, -pentaose, or -hexaose ?
xylohexaose + H2O
-
Aspergillus niger xylobiose + D-xylose + ?
-
?
xylohexaose + H2O
-
Aspergillus niger IA-0010 xylobiose + D-xylose + ?
-
?
xylopentaose + H2O
-
Aspergillus niger xylobiose + D-xylose
-
?
xylotetraose + H2O
-
Aspergillus niger 2 xylobiose
-
?
xylotetraose + H2O
-
Aspergillus niger IA-0010 2 xylobiose
-
?
xylotriose + H2O
-
Aspergillus niger xylobiose + D-xylose
-
?
xylotriose + H2O
-
Aspergillus niger IA-0010 xylobiose + D-xylose
-
?

Subunits

Subunits Comment Organism
? x * 23000, SDS-PAGE Aspergillus niger

Synonyms

Synonyms Comment Organism
XynB
-
Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
10 min, complete loss of activity Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Aspergillus niger