Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.8 extracted from

  • Bai, Y.; Wang, J.; Zhang, Z.; Yang, P.; Shi, P.; Luo, H.; Meng, K.; Huang, H.; Yao, B.
    A new xylanase from thermoacidophilic Alicyclobacillus sp. A4 with broad-range pH activity and pH stability (2010), J. Ind. Microbiol. Biotechnol., 37, 187-194.
    View publication on PubMed
Search for more literature for 3.2.1.8

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol activates at 1 mM Alicyclobacillus sp.

Cloned(Commentary)

Cloned (Comment) Organism
gene xynA4, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Alicyclobacillus sp.

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Alicyclobacillus sp.
Hg2+
-
 Alicyclobacillus sp.
Mn2+
-
 Alicyclobacillus sp.
Ni2+
-
 Alicyclobacillus sp.
SDS
-
 Alicyclobacillus sp.
Zn2+
-
 Alicyclobacillus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates at 1 mM Alicyclobacillus sp.
Co2+ activates at 1 mM Alicyclobacillus sp.
Cr3+ activates at 1 mM Alicyclobacillus sp.
Li+ activates at 1 mM Alicyclobacillus sp.
Mg2+ activates at 1 mM Alicyclobacillus sp.
additional information the enzyme is unaffected by 1-10 mM K+, Na+, and EDTA Alicyclobacillus sp.
Pb2+ activates at 1 mM Alicyclobacillus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42500
-
 x * 42500, about, sequence calculation Alicyclobacillus sp.

Organism

Organism UniProt Comment Textmining
Alicyclobacillus sp. D3VWB5 isolated from a hot spring in Baoshan City, Yunnan Province, China, gene xynA4
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Alicyclobacillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
birch wood xylan + H2O
-
 Alicyclobacillus sp. ?
-
 ?
oat spelt xylan + H2O
-
 Alicyclobacillus sp. ?
-
 ?

Subunits

Subunits Comment Organism
? x * 42500, about, sequence calculation Alicyclobacillus sp.

Synonyms

Synonyms Comment Organism
endo-1,4-beta-xylanase
-
 Alicyclobacillus sp.
XynA4
-
 Alicyclobacillus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Alicyclobacillus sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 65 XynA4 shows over 50% of maximal activity within this range Alicyclobacillus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 purified recombinant XynA4, 1 h, pH 7.0, over 90% activity remaining, half-life is 394 min Alicyclobacillus sp.
65
-
 purified recombinant XynA4, half-life is 17 min Alicyclobacillus sp.
70
-
 purified recombinant XynA4, half-life is 3 min Alicyclobacillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Alicyclobacillus sp.

pH Range

pH Minimum pH Maximum Comment Organism
3.8 9.4 XynA4 shows over 40% of maximal activity within this range Alicyclobacillus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
2.6 12 purified recombinant XynA4, 1 h, 37°C, over 80% remaining activity Alicyclobacillus sp.