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Literature summary for 3.2.1.8 extracted from

  • Barabote, R.D.; Parales, J.V.; Guo, Y.Y.; Labavitch, J.M.; Parales, R.E.; Berry, A.M.
    Xyn10A, a thermostable endoxylanase from Acidothermus cellulolyticus 11B (2010), Appl. Environ. Microbiol., 76, 7363-7366.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.8

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression in Escherichia coli Acidothermus cellulolyticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.35
-
 oat spelt xylan pH 6.0, 90°C Acidothermus cellulolyticus

Organism

Organism UniProt Comment Textmining
Acidothermus cellulolyticus
-
-
-
Acidothermus cellulolyticus 11B
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli by heat treatment at 65°C for 15 min and hydroxyapatite chromatography Acidothermus cellulolyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
birch wood xylan + H2O best substrate, thin-layer chromatography determination of reaction products, the major products from oat spelt and birchwood xylan have retention factor values between those of xylose and xylopentaose Acidothermus cellulolyticus ?
-
 ?
birch wood xylan + H2O best substrate, thin-layer chromatography determination of reaction products, the major products from oat spelt and birchwood xylan have retention factor values between those of xylose and xylopentaose Acidothermus cellulolyticus 11B ?
-
 ?
additional information Xyn10A is an endoxylanase. No activity with cellulose, carboxymethyl cellulose, and xanthan gum Acidothermus cellulolyticus ?
-
 ?
additional information Xyn10A is an endoxylanase. No activity with cellulose, carboxymethyl cellulose, and xanthan gum Acidothermus cellulolyticus 11B ?
-
 ?
oat spelt xylan + H2O thin-layer chromatography determination of reaction products, the major products from oat spelt and birchwood xylan have retention factor values between those of xylose and xylopentaose Acidothermus cellulolyticus ?
-
 ?
oat spelt xylan + H2O thin-layer chromatography determination of reaction products, the major products from oat spelt and birchwood xylan have retention factor values between those of xylose and xylopentaose Acidothermus cellulolyticus 11B ?
-
 ?

Synonyms

Synonyms Comment Organism
More the enzyme is a family 10 xylanase Acidothermus cellulolyticus
Xyn10A
-
 Acidothermus cellulolyticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
 Acidothermus cellulolyticus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 100 activity range, pH-dependent: at 100°C activity occurs at pH 4.0-6.0, at 60°C activity occurs at pH 5.0-8.0, overview Acidothermus cellulolyticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
 half-life is 1.5 h in presence of xylan Acidothermus cellulolyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Acidothermus cellulolyticus

pH Range

pH Minimum pH Maximum Comment Organism
4 8 activity range, at pH 8.0 activity occurs only up to 70°C, at pH 7.0 up to 90°C, at pH 4.0 activity occurs at 90-100°C, overview Acidothermus cellulolyticus