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Literature summary for 3.2.1.8 extracted from

  • Wang, C.; Chan, H.; Lin, H.; Shyu, Y.
    Production, purification and characterisation of a novel halostable xylanase from Bacillus sp. NTU-06 (2010), Ann. Appl. Biol., 156, 187-197.
    View publication on PubMed

Application

Application Comment Organism
industry xylanase is an important industrial enzyme used in the pulp and paper industry Bacillus sp. (in: Bacteria)
paper production xylanase is an important industrial enzyme used in the pulp and paper industry Bacillus sp. (in: Bacteria)

General Stability

General Stability Organism
43% of the enzyme activity is retained after 4 h in 20% sodium chloride Bacillus sp. (in: Bacteria)

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl required, optimal in 5% sodium chloride Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
x * 24000, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-
Bacillus sp. (in: Bacteria) NTU-06
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 36.7fold by anion exchange chromatography, gel filtration, and ultrafiltration, and a second step of gel filtration Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
312
-
purified enzyme, pH 8.0, 40°C, substrate birchwood xylan Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
birch wood xylan + H2O best substrate Bacillus sp. (in: Bacteria) ?
-
?
birch wood xylan + H2O best substrate Bacillus sp. (in: Bacteria) NTU-06 ?
-
?
oat spelt xylan + H2O
-
Bacillus sp. (in: Bacteria) ?
-
?
oat spelt xylan + H2O
-
Bacillus sp. (in: Bacteria) NTU-06 ?
-
?

Subunits

Subunits Comment Organism
? x * 24000, SDS-PAGE Bacillus sp. (in: Bacteria)

Synonyms

Synonyms Comment Organism
beta-1,4-endoxylanase
-
Bacillus sp. (in: Bacteria)
More the enzyme is a member of glycoside hydrolase family 11 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 50 purified enzyme, highly stable at Bacillus sp. (in: Bacteria)
70
-
4 h, 20% activity remaining Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Bacillus sp. (in: Bacteria)

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5 over 80% of maximal activity within this range Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 8 purified enzyme, 4 h, 80% activity remaining Bacillus sp. (in: Bacteria)
10
-
purified enzyme, 4 h, 47% activity remaining Bacillus sp. (in: Bacteria)

General Information

General Information Comment Organism
additional information the enzyme shows a high salt tolerance Bacillus sp. (in: Bacteria)