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Literature summary for 3.2.1.8 extracted from

  • Shin, J.; Choi, J.; Lee, O.; Kim, Y.; Lee, D.; Kwak, Y.; Kim, W.; Rhee, I.
    Thermostable xylanase from Streptomyces thermocyaneoviolaceus for optimal production of xylooligosaccharides (2009), Biotechnol. Bioprocess Eng., 14, 391-399.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
gene xynB, DNA and amino acid sequence determination and analysis,over expression in Escherichia coli strain BLR(DE3) Streptomyces thermocyaneoviolaceus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-1,4-xylan + H2O Streptomyces thermocyaneoviolaceus
-
xylo-oligosaccharides
-
?
beta-1,4-xylan + H2O Streptomyces thermocyaneoviolaceus M049
-
xylo-oligosaccharides
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces thermocyaneoviolaceus Q9RMM4 gene xynB
-
Streptomyces thermocyaneoviolaceus M049 Q9RMM4 gene xynB
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration Streptomyces thermocyaneoviolaceus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-1,4-xylan + H2O
-
Streptomyces thermocyaneoviolaceus xylo-oligosaccharides
-
?
beta-1,4-xylan + H2O from birchwood Streptomyces thermocyaneoviolaceus xylo-oligosaccharides
-
?
beta-1,4-xylan + H2O
-
Streptomyces thermocyaneoviolaceus M049 xylo-oligosaccharides
-
?
beta-1,4-xylan + H2O from birchwood Streptomyces thermocyaneoviolaceus M049 xylo-oligosaccharides
-
?

Synonyms

Synonyms Comment Organism
xylanase
-
Streptomyces thermocyaneoviolaceus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
native enzyme Streptomyces thermocyaneoviolaceus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 native enzyme, activity range, profile, overview Streptomyces thermocyaneoviolaceus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 70 purified enzyme, 1 h, 65% activity remaining at 70°C, completely stable at 30-60°C, overview Streptomyces thermocyaneoviolaceus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
native enzyme Streptomyces thermocyaneoviolaceus

pH Range

pH Minimum pH Maximum Comment Organism
3 9 native enzyme, activity range, profile, overview Streptomyces thermocyaneoviolaceus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 10.5 purified enzyme, stable at pH 4.5-10.5, 30% remaining at pH 3.0, overview Streptomyces thermocyaneoviolaceus