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Literature summary for 3.2.1.8 extracted from

  • Al Balaa, B.; Brijs, K.; Gebruers, K.; Vandenhaute, J.; Wouters, J.; Housen, I.
    Xylanase XYL1p from Scytalidium acidophilum: site-directed mutagenesis and acidophilic adaptation (2009), Biores. Technol., 100, 6465-6471.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloning in Escherichia coli strain DH10B, overexpression of wild-type and mutant enzymes in Pichia pastoris strain X-33 Acidomyces acidophilus

Protein Variants

Protein Variants Comment Organism
D60N site-directed mutagenesis, the mutant shows an increased pH optimum and over 90% reduced specific activity compared to the wild-type enzyme Acidomyces acidophilus
D60N/E141A site-directed mutagenesis, the mutant shows an increased pH optimum and over 90% reduced specific activity compared to the wild-type enzyme Acidomyces acidophilus
D60N/Y35W site-directed mutagenesis, the mutant shows an increased pH optimum and over 90% reduced specific activity compared to the wild-type enzyme Acidomyces acidophilus
E141A site-directed mutagenesis, the mutant shows an increased pH optimum, slightly reduced thermostability, 50% increase in specific activity at pH 4.0, and an overall increased catalytic efficiency compared to the wild-type enzyme Acidomyces acidophilus
Y35W site-directed mutagenesis, the mutant shows an increased pH optimum and 64% reduced specific activity compared to the wild-type enzyme Acidomyces acidophilus
Y35W/E141A site-directed mutagenesis, the mutant shows an increased pH optimum and 46% reduced specific activity compared to the wild-type enzyme Acidomyces acidophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics, wild-type and mutant enzymes, overview, wild-type enzyme Km for beechwood xylan is 14.8 mg/ml Acidomyces acidophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-1,4-xylan + H2O Acidomyces acidophilus
-
?
-
?

Organism

Organism UniProt Comment Textmining
Acidomyces acidophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant enzymes from Pichia pastoris strain X-33 by TAXI inhibitor affinity chromatography Acidomyces acidophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-1,4-xylan + H2O
-
Acidomyces acidophilus ?
-
?
beta-1,4-xylan + H2O xylan from beechwood Acidomyces acidophilus ?
-
?
additional information residues Asp60, Tyr35 and Glu141 play a role in the pH-dependent activity of xylanase XYL1p, overview Acidomyces acidophilus ?
-
?

Synonyms

Synonyms Comment Organism
XYL1p
-
Acidomyces acidophilus
xylanase
-
Acidomyces acidophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50 60 wild-type and mutant enzymes Acidomyces acidophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 70
-
Acidomyces acidophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
68.2
-
beta-1,4-xylan pH 4.0, 56°C, beechwood xylan Acidomyces acidophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2
-
wild-type enzyme Acidomyces acidophilus
3.7
-
mutant Y35W Acidomyces acidophilus
4
-
mutant E141A Acidomyces acidophilus
4
-
mutant Y35W/E141A Acidomyces acidophilus
4.1
-
mutant D60N Acidomyces acidophilus
4.4
-
mutant D60N/Y35W Acidomyces acidophilus
4.7
-
mutant D60N/E141A Acidomyces acidophilus