Cloned (Comment) | Organism |
---|---|
cloning in Escherichia coli strain DH10B, overexpression of wild-type and mutant enzymes in Pichia pastoris strain X-33 | Acidomyces acidophilus |
Protein Variants | Comment | Organism |
---|---|---|
D60N | site-directed mutagenesis, the mutant shows an increased pH optimum and over 90% reduced specific activity compared to the wild-type enzyme | Acidomyces acidophilus |
D60N/E141A | site-directed mutagenesis, the mutant shows an increased pH optimum and over 90% reduced specific activity compared to the wild-type enzyme | Acidomyces acidophilus |
D60N/Y35W | site-directed mutagenesis, the mutant shows an increased pH optimum and over 90% reduced specific activity compared to the wild-type enzyme | Acidomyces acidophilus |
E141A | site-directed mutagenesis, the mutant shows an increased pH optimum, slightly reduced thermostability, 50% increase in specific activity at pH 4.0, and an overall increased catalytic efficiency compared to the wild-type enzyme | Acidomyces acidophilus |
Y35W | site-directed mutagenesis, the mutant shows an increased pH optimum and 64% reduced specific activity compared to the wild-type enzyme | Acidomyces acidophilus |
Y35W/E141A | site-directed mutagenesis, the mutant shows an increased pH optimum and 46% reduced specific activity compared to the wild-type enzyme | Acidomyces acidophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics, wild-type and mutant enzymes, overview, wild-type enzyme Km for beechwood xylan is 14.8 mg/ml | Acidomyces acidophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-1,4-xylan + H2O | Acidomyces acidophilus | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidomyces acidophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Pichia pastoris strain X-33 by TAXI inhibitor affinity chromatography | Acidomyces acidophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-1,4-xylan + H2O | - |
Acidomyces acidophilus | ? | - |
? | |
beta-1,4-xylan + H2O | xylan from beechwood | Acidomyces acidophilus | ? | - |
? | |
additional information | residues Asp60, Tyr35 and Glu141 play a role in the pH-dependent activity of xylanase XYL1p, overview | Acidomyces acidophilus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
XYL1p | - |
Acidomyces acidophilus |
xylanase | - |
Acidomyces acidophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 60 | wild-type and mutant enzymes | Acidomyces acidophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 70 | - |
Acidomyces acidophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
68.2 | - |
beta-1,4-xylan | pH 4.0, 56°C, beechwood xylan | Acidomyces acidophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.2 | - |
wild-type enzyme | Acidomyces acidophilus |
3.7 | - |
mutant Y35W | Acidomyces acidophilus |
4 | - |
mutant E141A | Acidomyces acidophilus |
4 | - |
mutant Y35W/E141A | Acidomyces acidophilus |
4.1 | - |
mutant D60N | Acidomyces acidophilus |
4.4 | - |
mutant D60N/Y35W | Acidomyces acidophilus |
4.7 | - |
mutant D60N/E141A | Acidomyces acidophilus |