Application | Comment | Organism |
---|---|---|
paper production | since XynA is thermostable and has no cellulase activity it can be potentially used for pretreatment of paper pulp before bleaching | Paecilomyces sp. 'thermophila' |
Cloned (Comment) | Organism |
---|---|
gene xynA, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli strain DH5alpha, expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) | Paecilomyces sp. 'thermophila' |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics, Km values of XynA for birchwood xylan, beechwood xylan, and oat-spelt xylan are 4.4 mg/ml, 3.6 mg/ml, and 9.7 mg/ml, respectively | Paecilomyces sp. 'thermophila' |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26132 | - |
x * 26132, XynA, sequence calculation, x * 28000, XynA, SDS-PAGE | Paecilomyces sp. 'thermophila' |
28000 | - |
x * 26132, XynA, sequence calculation, x * 28000, XynA, SDS-PAGE | Paecilomyces sp. 'thermophila' |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Paecilomyces sp. 'thermophila' | beta-1,4-endoxylanases are enzymes which randomly hydrolyse the beta-1,4-glycosidic bonds in the xylan backbone | ? | - |
? | |
additional information | Paecilomyces sp. 'thermophila' J18 | beta-1,4-endoxylanases are enzymes which randomly hydrolyse the beta-1,4-glycosidic bonds in the xylan backbone | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paecilomyces sp. 'thermophila' | D1G4K3 | gene XynA | - |
Paecilomyces sp. 'thermophila' J18 | D1G4K3 | gene XynA | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the protein sequence contains one possible N-glycosylation site, consensus Asn-Xaa-Ser/Thr, at position 25 and two potential O-glycosylation sites at positions 26/27, respectively | Paecilomyces sp. 'thermophila' |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme 48fold from Escherichia coli by nickel affinity chromatography | Paecilomyces sp. 'thermophila' |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
706.1 | - |
purified recombinant XynA | Paecilomyces sp. 'thermophila' |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-1,4-xylan + H2O | xylan from birchwood, beechwood, and oat spelt | Paecilomyces sp. 'thermophila' | ? | - |
? | |
beta-1,4-xylan + H2O | xylan from birchwood, beechwood, and oat spelt | Paecilomyces sp. 'thermophila' J18 | ? | - |
? | |
additional information | beta-1,4-endoxylanases are enzymes which randomly hydrolyse the beta-1,4-glycosidic bonds in the xylan backbone | Paecilomyces sp. 'thermophila' | ? | - |
? | |
additional information | the enzyme has an endohydrolytic mode of action and can hydrolyse xylotriose to xylobiose through transglycosylation | Paecilomyces sp. 'thermophila' | ? | - |
? | |
additional information | beta-1,4-endoxylanases are enzymes which randomly hydrolyse the beta-1,4-glycosidic bonds in the xylan backbone | Paecilomyces sp. 'thermophila' J18 | ? | - |
? | |
additional information | the enzyme has an endohydrolytic mode of action and can hydrolyse xylotriose to xylobiose through transglycosylation | Paecilomyces sp. 'thermophila' J18 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 26132, XynA, sequence calculation, x * 28000, XynA, SDS-PAGE | Paecilomyces sp. 'thermophila' |
Synonyms | Comment | Organism |
---|---|---|
beta-1,4endoxylanase | - |
Paecilomyces sp. 'thermophila' |
xylanase | - |
Paecilomyces sp. 'thermophila' |
XynA | - |
Paecilomyces sp. 'thermophila' |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
recombinant His-tagged enzyme | Paecilomyces sp. 'thermophila' |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
recombinant His-tagged enzyme, 30 min, over 90% activity remaining | Paecilomyces sp. 'thermophila' |
70 | - |
recombinant His-tagged enzyme, half-life is 174.8 min | Paecilomyces sp. 'thermophila' |
75 | - |
recombinant His-tagged enzyme, half-life is 137.9 min | Paecilomyces sp. 'thermophila' |
85 | - |
recombinant His-tagged enzyme, 30 min, over 60% activity remaining, half-life is 68.2 min | Paecilomyces sp. 'thermophila' |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
recombinant His-tagged enzyme | Paecilomyces sp. 'thermophila' |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Paecilomyces sp. 'thermophila' | sequence calculation | - |
5.47 |